胸腺修饰诱导同种异体静脉移植的免疫耐受研究  

Inducing transplantation immune tolerance to rats vein by intrathymic injection of allogenic antigen

作　　者：孟庆刚[1] 孙丕云[1] 岳伟杰[1] 胡成乙[2] 王立群[3] 

机构地区：[1]哈尔滨医科大学附属第四医院骨外科,150001 [2]哈尔滨医科大学附属第四医院病理科,150001 [3]哈尔滨医科大学基础医学院免疫教研室

出　　处：《中华实验外科杂志》2009年第7期895-897,共3页Chinese Journal of Experimental Surgery

基　　金：黑龙江省自然科学基金资助项目（IY2007-55）

摘　　要：目的观察大鼠胸腺内注射异基因抗原在同种异体异基因股静脉移植免疫耐受中的作用。方法将48只SD大鼠随机分为4组：自体股静脉移植组（A组）、异体股静脉移植组（B组）、异体股静脉移植免疫抑制剂组（C组）、胸腺内注射供体组织相容性（MHC）抗原后移植组（D组）。于2周后进行影像学、组织学、免疫学检测。结果组织学检测结果显示：D组、C组急性排斥反应损伤较轻，B组血管壁的各层结构破坏最重，可见大量炎性细胞浸润。B组受体大鼠血清干扰素（IFN）-γ浓度为（86．707±10．928）ng／L，显著高于A、C、D组[（29．328±4．170）、（69．076±8．059）、（63．355±4．895）ng／L，P〈0．05]；B组受体大鼠血清白细胞介素（IL）-4浓度为（23．656±3．369）ng／L，显著低于C、D组[（29．425±4．174）、（31．000±4．659）ng／L，P〈0．05]。结论胸腺内注射异基因NHC抗原可诱导大鼠对同种异体血管移植的特异性免疫耐受。Objective To investigate the possibility of inducing immune tolerance to vein transplantation in rats by intrathymic injection of allogene. Methods MHC antigen extracted from splenic cells of donor Wistar rats was intrathymically injected to recipients SD rats, and donor Wistar femur vein was transplanted after 2 weeks. Forty-eight SD rats were randomly divided into 4 groups ： group A （ femur vein autograft）, group B （ femur vein allograft）, group C （ femur vein allograft with use of immunosuppressant）, group D （intrathymus injection）. Imaging, histology and immunological assays of these groups were carried out 2 weeks after the transplantation. Results Histologic parameters that were tested were better in group D than those in group B. The concentration of serum IFN-γ in group B was significantly higher than that in groups A,C and D [ （86. 707 ± 10.928） ng/L vs （29.328 ±4. 170）, （69.076 ± 8. 059） and （63. 355 ± 4.895） ng/L respectively, P 〈 0. 05 ]. The concentration of serum IL-4 in group B was obviously lower than in groups C and D [ （23. 656 ± 3. 369） ng/L vs （29. 425 ± 4. 174） and （31. 000 ± 4.659） ng/L respectively,P 〈 0.05 ]. Conclusion Intrathymus injection of allogenie antigen might induce specific immune tolerance to femur vein transplantation in rats.

关 键 词：胸腺 抗原 静脉移植 免疫耐受 

分 类 号：R654.3[医药卫生—外科学] R392.4[医药卫生—临床医学]