H2O2诱导的肝星状细胞凋亡后胶原的变化  

Changes of collagens after apoptosis of HSC induced by H2O2

作　　者：洪峰[1] 王爱民[1] 杨晓明[2] 耿焱[1] 张莉[1] 

机构地区：[1]解放军第252医院消化科,河北保定071000 [2]军事医学科学院放射医学研究所

出　　处：《中华实验外科杂志》2009年第7期902-903,共2页Chinese Journal of Experimental Surgery

摘　　要：目的观察Ⅰ型胶原（COLI）和Ⅲ型胶原（COLⅢ）在H2O2诱导的肝星状细胞（HSC）凋亡后的变化。方法用流式细胞术检测100nmol／L的H2O2诱导HSC不同程度的凋亡；应用半定量逆转录-聚合酶链反应（RT—PCR）检测细胞内COLⅠ、COLⅢmRNA的表达及变化。结果用100nmol／L H2O2诱导HSC凋亡（凋亡率分别5．86％、58．55％、71．98％），COLⅠ、COLⅢ在活化的HSC—T6细胞内均高表达，且伴随凋亡的增加，表达急剧下降，COLⅠ的变化更明显。结论诱导HSC凋亡可减少胶原基因的表达。Objective To explore the changes of collagen （COL） Ⅰ and COLⅢ after apoptosis of HSC induced by H2O2. Methods Different apoptosis rate of HSC-T6 induced by H2O2 （ 100 nmol/L） was evaluated by flow cytometry. RT-PCR was used to detect the gene expression levels of COL Ⅰ and COL Ⅲ. Results Apoptosis of HSC was induced by 100 nmol/L H2O2 with the poptosis rate being 5.86%, 58.55 % , and 71.98 % , respectively. The COL Ⅰ and COL Ⅲ were highly expressed in activated HSC, and decreased sharply as apoptosis increased. The changes in gene expression of COL Ⅰ were much more obvious than those of COL Ⅲ. Conclusion Inducing apoptosis of HSC may decrease the gene expression of collagens.

关 键 词：肝星状细胞 脱噬作用 胶原 基因表达 

分 类 号：R285.5[医药卫生—中药学]