大鼠海马胶质细胞培养牵张损伤模型的建立  

The establishment of an in vitro model of stretch-induced injury of cultured rat hippocampal astrocytes

作　　者：郭建毅[1] 钟春龙[1] 张风江[1] 范东[1] 江基尧[1] 罗其中[1] 

机构地区：[1]上海交通大学医学院附属仁济医院神经外科,上海200127

出　　处：《立体定向和功能性神经外科杂志》2009年第3期152-155,共4页Chinese Journal of Stereotactic and Functional Neurosurgery

基　　金：国家自然科学基金项目(编号:30500525;30670719);上海市科委基础重点研究计划项目(编号:07JC14038);上海市青年科技启明星计划项目(编号:05QMX1428)

摘　　要：目的建立大鼠海马胶质细胞培养牵张损伤模型。方法提取出生时间24h到48h的SD大鼠海马组织行星形胶质细胞培养,采用CICⅡ型细胞损伤装置、根据Ellis方法建立改良的大鼠海马胶质细胞体外牵张损伤模型,损伤程度分轻、中、重三级。对照组不予损伤。分别在2h和24h检测细胞乳酸脱氢酶释放量,并通过碘化丙啶荧光染色观察细胞损伤情况。结果细胞培养液中乳酸脱氢酶释放量随着损伤程度加重而增高。PrI红染细胞数随着牵张损伤的程度增高而增加。结论本实验建立的牵张损伤模型使用方便,可重复性好,适于进行神经细胞机械性体外损伤的研究。Objective To establish an in vitro model of stretch--induced injury of cultured rat hippocampal astrocytes. Methods Astrocytes were extracted from 24h-- to 48h--old rats＇hippocampal tissue. The modified in vitro model was established by Ellis＇means, using an CIC Ⅱinjury system. The injury was graded as mild, moderate, severe respectively. The control groups did not take injury. Cell injury was assessed by measuring the releasement of LDH in the culture medium at the time points of 2h and 24h. Cell injury was also assessed by Propidium Iodide Staining. Results The releasement of LDH in the culture medium and the number of red--stained cells increased as injury aggravated. Conclusion This model is easy to operate and has a good control and measurement criteria. It is suitable for in vitro mechanical injury studies of brain--derived ceils.

关 键 词：星形胶质细胞 牵张损伤 细胞培养 体外模型 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]