胸腺肽-β4在小鼠胚胎成纤维细胞中的定位规律  

The Distribution of Thymosin-β4 in Mouse Embryonic Fibroblasts

作　　者：张岩[1] 李树峰[1] 李宁[1] 杨彩荣[1] 郑可佳[1] 严云勤[1] 

机构地区：[1]东北农业大学动物细胞与发育生物学教研室,哈尔滨150030

出　　处：《细胞生物学杂志》2009年第3期414-418,共5页Chinese Journal of Cell Biology

摘　　要：胸腺肽-β4(thymosin-β4,Tb4)是一种重要的G-actin活性遮蔽因子,其能够在细胞微丝活动中对G-actin的活性进行调节。虽然许多研究报道了Tb4具有广泛的生理功能与作用,但其在细胞中的定位规律迄今还没有达成共识。因此本研究选用小鼠胚胎成纤维细胞(mouse embryonic fibroblasts,MEFs)作为实验模型,以免疫荧光组织化学技术为主要实验方法,以激光共聚焦扫描显微镜为主要观测工具,研究了Tb4在MEFs中的定位规律。结果显示Tb4在MEFs贴壁、生长、增殖过程和特定细胞周期中有着显著的核、质定位变化规律,细胞形态变化和细胞所处周期两大因素能够决定Tb4在MEFs中的定位类型。As an important G-actin sequestering factor, thymosin-β4 （Tb4） regulates the G-actin activity during many cellular microfilaments events. Hitherto, serious of reports have confirmed that Tb4 has broad cellular and physiological functions, but the discipline of cellular location have never reached a consensus. Thus, we chose mouse embryonic fibroblasts （MEFs） as experimental model, and utilized immuno-fluorescence-histochemistry technology as the chief method, while confocal laser scanning microscope as the main instrument. The results showed that the distributive type of Tb4 changed regularly in the processes of MEFs＇ adhesion, growth, proliferation and special cell cycle. In conclusion, the distributive type of Tb4 in MEFs is determined by the special cell cycle and the transformation of cellular morphology, which always accompanied with intensive microfilaments polymerization or depolymerazation.

关 键 词：胸腺肽-β4 小鼠胚胎成纤维细胞 微丝 免疫荧光组织化学 激光共聚焦扫描显微 

分 类 号：Q26[生物学—细胞生物学]