Nodal在大鼠卵巢各不同发育时期卵泡中的表达模式  

The Expression of Nodal in Rat Ovarian Follicles at Different Developmental Stages

作　　者：闫利平[1] 李闯[2] 郭炳冉[1] 

机构地区：[1]曲阜师范大学生命科学学院,曲阜273165 [2]中国科学院动物研究所计划生育生殖生物学国家重点实验室,北京100101

出　　处：《细胞生物学杂志》2009年第3期419-423,共5页Chinese Journal of Cell Biology

基　　金：曲阜师范大学"十一五"计划省级重点建设项目资助~~

摘　　要：为探索在卵泡发育过程中Nodal的表达规律及其可能的作用机制,本文应用免疫组化方法检测了Nodal在大鼠不同发育时期卵泡中的表达情况。在未成年大鼠中注射孕马血清(PMSG)12、24、36和48h后分别收集卵巢,进行石蜡切片;分别选取相邻的连续切片,苏木素-伊红(HE)染色观察PMSG诱导后大鼠卵泡不同发育阶段的形态;免疫组化方法检测Nodal的表达情况。结果显示,Nodal在腔前卵泡中未见表达。随着卵泡的增长,Nodal在有腔卵泡的近卵泡腔颗粒细胞(granulosa cells,GC)的细胞质中表达,且随着卵泡腔的增大其表达也有所增强,说明Nodal在卵泡腔形成和卵泡生长过程中可能发挥了一定的作用。To examine the expression and the role of Nodal in rat follicles at different stages of development. Pre-mature rats were injected with PMSG （10 IU/rat）, and ovaries were collected at 12, 24, 36 and 48 h after injection; Serial sections were prepared for each ovary; The structures of rat ovarian follicles were shown by HE staining after PMSG injection, and the localization of Nodal in rat ovaries was determined by immunohistochemistry. The results showed that Nodal was mainly distributed in granulosa cells close to the follicle cavity. It is suggested that Nodal may play a vital role in the formation of follicle cavity.

关 键 词：NODAL 卵巢 卵泡腔 颗粒细胞 大鼠 

分 类 号：Q492[生物学—生理学]