咖啡酸及其衍生物对培养大鼠大脑皮层神经细胞的影响  

Effect of caffeic acid and its derivative on rat cerebral cortex neurons

作　　者：盛艳梅[1,2] 张静[2] 罗维早[3] 张艺[2] 

机构地区：[1]成都医学院药学院,四川成都610083 [2]成都中医药大学药学院,四川成都610075 [3]重庆市中药研究院,重庆400065

出　　处：《中国医院药学杂志》2009年第12期969-971,共3页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:30672602)

摘　　要：目的:考察灯盏细辛中咖啡酸及其衍生物对体外培养大鼠大脑皮层神经细胞存活的影响。方法:取出生1d内的乳鼠大脑皮层制成细胞悬液,接种于经多聚赖氨酸包被的96孔板中。于培养6d后,分别加入培养液及不同浓度的咖啡酸类药物,继续培养1d,采用四甲基偶氮唑盐(MTT)比色法测量其存活细胞的吸收值,同时行神经特异性烯醇化酶(NSE)检查。结果:NSE检查结果表明,培养6d的存活细胞大部分均为神经元。与空白对照组比较,咖啡酸(100,400mg·L-1)、咖啡酸甲酯(4,20mg·L-1)、咖啡酸乙酯(20mg·L-1)、3,4-二乙酰基咖啡酸(4,20,100mg·L-1)的存活细胞数均明显增加(P<0.05,P<0.01)。结论:各有效成分均能不同程度促进体外培养的大脑皮层神经细胞存活,且以3,4-二乙酰基咖啡酸活性较强。OBJECTIVE To explore the effect of caffeic acid and its derivate from Erigeron breviscapus （Vant.） Hand. Mazz. on rat cerebral neurons in vitro. METHODS The cerebral cortex of post-natal under 1 day Sprague-Dawley rats was dissociated into cell suspension. The cell suspension was incubated in 96-well culture plates covered with polylysine in each well. After cultivated for 6 days, reagents were added to the cultures in order, continue to cultivate 1 day. Then,the OD of living cells in each well was measured by MTT assay. Some of the 6-day culture cells were stained with neuronal specific nolase （NSE） antibody. RESULIS Most of the living cells were cerebral neurons by NSE. Compared with control group, the neuron survival in high concentration of caffeic acid（100,400 mg·L^-1）, caffeic acid methyl ester（4,20mg·L^-1）, caffeic acid ethyl ester（20mg·L^-1） ,3,4-diacetyl caffeic acid（4, 20,100 mg·L^-1 ）were significantly higher than those in control group（P〈0. 05, 0. 01）. CONCLUSION The active components can all promote cerebral cortex neurons to live in different degree, and 3,4-diacetyl caffeic acid is the active one.

关 键 词：灯盏细辛 咖啡酸及其衍生物 神经细胞培养 

分 类 号：R285.6[医药卫生—中药学]