超声辐照载羟基喜树碱微泡对肝癌SMMC-7721细胞凋亡及相关蛋白表达的影响  

Effects of ultrasound irradiating hydroxycamptothecin-loaded microbubbles on apoptosis and expression of related protein in human hepatoma SMMC-7721 cells

作　　者：朱婷[1] 孙彤[1] 王志刚[2] 李攀[2] 严思静[2] 

机构地区：[1]重庆医科大学附属第一医院超声科,400016 [2]重庆医科大学超声影像学研究所

出　　处：《中华超声影像学杂志》2009年第6期529-532,共4页Chinese Journal of Ultrasonography

基　　金：国家863科技攻关项目（2006AA02Z4F0）;国家自然科学基金重点项目（30430230）

摘　　要：目的研究超声辐照载羟基喜树碱微泡对肝癌SMMC-7721细胞凋亡的影响，并探讨其诱导细胞凋亡的分子机制。方法SMMC7721细胞随机分为6组，即对照组、空白脂质微泡＋超声组、载羟基喜树碱微泡组、羟基喜树碱组、羟基喜树碱＋超声组及载羟基喜树碱微泡＋超声组。采用AnnexinV—FITC／PI双染法和TUNEL法检测细胞凋亡状况；免疫细胞化学法检测Bcl-2、Bax蛋白表达。结果载羟基喜树碱微泡＋超声组用AnnexinVFITC／PI检测细胞早期凋亡率为（12．18±1．38）％；TUNEL法显示染成棕黄色的凋亡细胞，细胞中晚期凋亡率为（34．25±1．83）％；免疫细胞化学法检测Bcl-2蛋白表达下降，Bax蛋白表达升高，Bcl-2／Bax比值明显下降。以上结果与其他组的检测结果相比，差异具有统计学意义。结论超声辐照载羟基喜树碱微泡能明显诱导肝癌SMMC-7721细胞凋亡，其机制可能与降低Bcl-2／Bax比值有关。Objective To investigate the effects of ultrasound irradiating hydroxycamptothecin （HCPT）-loaded microhubbles on apoptosis in human hepatoma SMMC-7721 cells,and to clarify its probable mechanism. Methods The SMMC-7721 cells were randomly divided into six groups as follows： control group, blank liposome microbubbles plus ultrasound, HCPT loaded mierobubbles, HCPT, HCPT plus ultrasound,and HCPT-loaded microbubbles plus ultrasound. Apoptosis was ascertained by Annexin V-Fitc/ PI and TUNEL methods. The expression of Bcl 2 and Bax protein was detected by immunocytochemistry staining technique. Results In the group of HCPT loaded microbubbles plus ultrasound,the apoptosis rate at early term was found to reach （12. 18± 1.38）% by Annexin V-Fitc/PI assay, brown colored positive apoptotic cells were observed and the apoptosis rate at advanced term was found to reach （34.25 ± 1.83） %with TUNEL method. The expression of Bcl-2 was found to decrease, the expression of Bax increase, and the ratio of Bcl- 2/bax significantly decrease by immunocytochemistry staining technique in the same group. Differences with other groups were significant. Conclusions The method of ultrasound irradiating HCPT loaded microbubbles can notability induce human hepatoma SMMC-7721 cells apoptosis. The decrease in the ratio of Bcl-2 to Bax might be responsible for cells apoptosis.

关 键 词：超声检查 微气泡 肝肿瘤 实验性 喜树碱 细胞凋亡 

分 类 号：R686[医药卫生—骨科学]