纳米脱钙骨基质的制备及其性能检测  

Preparation of nano DBM and its properties

作　　者：黄凯[1] 陈雄生[1] 贾连顺[1] 邵将[1] 朱巍[1] 严望军[1] 房雷[1] 

机构地区：[1]第二军医大学长征医院骨科,上海市凤阳路415号200003

出　　处：《中国矫形外科杂志》2009年第13期1017-1019,共3页Orthopedic Journal of China

基　　金：上海市科委基金资助项目(编号:054119641;0852nm03100)

摘　　要：[目的]通过MICROS超细粉碎机制备同种异体纳米脱钙骨基质(DBM),观察纳米DBM结构特征,研究DBM纳米化工艺及其作为骨移植替代物的生物相容性。[方法]采用改良Urist法制备同种异体脱钙骨基质,液氮冷冻球磨机将块状DBM预粉碎,使用MICROS超细粉碎机进一步研磨粉碎制备纳米DBM。电镜扫描观察其结构,按照我国卫生部《生物材料和医疗器材生物学评价的技术要求》中的标准,对纳米DBM进行急性毒性实验、热原实验、溶血实验等检测。[结果]制备颗粒直径在50～200纳米的脱钙骨基质,生物相容性检测无毒性,无热源性,不引起溶血反应,纳米DBM具有良好的生物相容性。[结论]纳米DBM可在低温或控制温度条件下制备,是一种无毒、无刺激,不含热源、不引起免疫排斥反应的生物材料,具有良好生物相容性。[ Objective] To observe the structure characteristics of nano decalcified bone matrix （DBM） produced by MI- CROS super fine mill, and to study the nano - technologies of DBM and the biocompatibility of nano - DBM as bone graft substi- tutes. [ Methods ] Improved Urist method was used to produce DBM. The pieces of DBM was pro - porphyrizated by liquid ni- trogen frozen ball mill equipment. The DBM powder were further porphyrizated by MICROS super fine mill to be nano - DBM. The structure of nano - DBM was observed by electron microscope. The experiments on acute toxicity, pyrogen and hemolysis were performed according to theTechnical Evaluation Standards of Biomedical Materials and Medical Instruments promulgated by Chinese Ministry of Health. [ Results] The DBM was porphyrizated to the size of 50 -200 nm in diameter. No toxicity, pyro- gen nor hemolysis of nano - DBM was noted. Nano - DBM exhibited excellent biocompatibility. [ Conclusion ] Nano - DBM produced under the conditions of low or controlled temperature, is a kind of biomaterial which is avirulent, nonirritant, nonpyro- genic, nonimmunological reaction and reveals good biocompatibility.

关 键 词：纳米脱钙骨基质 MICROS超细粉碎机 显微特征 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]