卡巴胆碱对烧伤延迟复苏大鼠免疫功能的影响  

Effect of carbachol on the expression of Ia on monocyte and lymphocyte apoptosis rate of the burned rats with delayed resuscitation

作　　者：于燕[1] 邹曰坤[2] 杨红明[3] 柴家科[3] 胡森[3] 盛志勇[3] 

机构地区：[1]海军总医院医学整形科,北京100037 [2]海军总医院ICU [3]解放军总医院第一附属医院全军烧伤研究所

出　　处：《中国医师杂志》2009年第6期727-729,共3页Journal of Chinese Physician

基　　金：首都医学发展科研基金联合攻关项目（2002-1010）

摘　　要：目的探讨卡巴胆碱对烧伤延迟复苏大鼠单核细胞Ia抗原、外周血淋巴细胞凋亡率及促炎细胞因子肿瘤坏死因子（TNF）-α、白介素（IL）-6的影响。方法104只成年雄性Wistar大鼠制作30％m度烧伤，延迟复苏模型。大鼠随机分为正常对照组、单纯烫伤组和卡巴胆碱治疗组，分别于6h、1、2、3、7、14d时间点处死动物并取血，流式细胞仪检测单核细胞la抗原表达率和淋巴细胞凋亡率，Elisa法检测TNFα、IL-6水平。结果大鼠烧伤延迟复苏后单核细胞Ia抗原表达率降低。其中6h、和1d时间点Ia抗原表达率严重降低，2d开始有所恢复，但仍明显低于正常大鼠（P〈0．01）。卡巴胆碱治疗组6h、1、2和3d时间点单核细胞Ia抗原表达率与单纯烫伤组比较明显提高（P〈0．01）。大鼠烫伤延迟复苏后淋巴细胞凋亡率在整个观察期与对照组比较均增高（P〈0．01）。给予卡巴胆碱后在6h、1、2和3d时间点与单纯烫伤组比较可见凋亡明显下降（P〈0．05）。烧伤延迟复苏后大鼠血浆TNF-α和IL-6与正常对照组比较明显升高（P〈0．01），应用卡巴胆碱后可见6h、1、2和3d时间点TNFα和IL-6含量与单纯烧伤组比较明显降低（P〈0．01）。结论烧伤延迟复苏后，单核细胞Ia抗原表达率降低，淋巴细胞凋亡率增加，促炎细胞因子增加，免疫功能障碍。卡巴胆碱可提高烫伤延迟复苏大鼠的免疫功能。Objective To explore the changing trend of Ia on monocyte, lymphocyte apoptosis rate, TNF-α and IL-6 in abdominal aorta of burned rats with delayed resuscitation and the influence of application of carbachol on them. Methods Adult male Wistar rats were randomly divided into normal control group（ n = 8 ）, scald group（ n = 48 ） and scald with carbachol treatment group（ n = 48 ）. In latter two groups, rats were inflicted with 30% total body surface area （TBSA） full-thickness scald and delayed fluid resuscitation. All scald rats were sacrificed at the 6th hours or 1st, 2nd, 3rd, 7th, 14th day after scald, with 8 rats at each time point. Expression of Ia antigen on monocyte and lymphocyte apoptosis rate were determined by direct immunofluoreseenee on a flow cytometer, and TNF-α and IL-6 was measured by ELISA. Results Expression of Ia on monocyte was obviously lower than that of controls. The lowest levels were recorded on the 6th hours and 1 st day after scald. Subsequently, Ia was elevated gradually, but still lower than that of normal rats（ P 〈 0. 01 ）. After administration of carbaehol, Ia expression was obviously promoted, compared with the simple scald group （ P 〈 0. 01 ）. Lymphocyte apoptosis rate, TNF-α and IL-6 was higher than that of controls（ P 〈 0. 01 ）. After administration of cabachol, , lymphocyte apoptosis rate and TNF-α and IL-6 was obviously down-regulated on the 6th hours, 1st day, 2nd day and 3rd day after scald injury, compared with the simple scald group （ P 〈0. 01 or 0. 05）. Conclusion After severe burn with delayed fluid resuscitation, there is a low Ia expression, high lymphocyte apoptosis rate and increased releasing of proinflammatory cytokine. Immune function was suppressed. Carbachol could improve the immune function of scald rats with delayed fluid resuscitation.

关 键 词：卡巴胆碱 药理学 烧伤 药物疗法 免疫学 

分 类 号：R6[医药卫生—外科学]