P13K、P38MAPK及ERK1／2抑制剂对EGF诱导的血管平滑肌细胞迁移的实验研究  

Effects of the inhibitors of PI3K, P38 MAPK and ERK1/2 on the migration of EGF-induced vascular smooth muscle cells

作　　者：张复贵[1,2] 闵新文[1] 曾秋棠[2] 陈龙菊[3] 姜铧[4] 

机构地区：[1]郧阳医学院附属东风总医院心内科,湖北十堰442000 [2]华中科技大学附属协和医院心内科 [3]郧阳医学院解剖学教研室 [4]郧阳医学院附属太和医院血液内科

出　　处：《中国医师杂志》2009年第6期742-744,共3页Journal of Chinese Physician

摘　　要：目的探讨P13K抑制剂Wortmannin、P38MAPK抑制剂SB202190及ERK1／2抑制剂PD98059对表皮生长因子（EGF）诱导的大鼠主动脉平滑肌细胞（VSMCs）迁移的影响。方法以1％FCS血清孵育为对照，用EGF（10ng／ml）刺激，平行加入PD98059（PD组）、SB202190（SB组）和Wortmannin（WT），划痕损伤实验分析VSMCs迁移情况。结果划痕后24h，EGF组VSMCs迁移较对照组明显（P〈0．01），WT组、SB组及PD纽与EGF组相比都明显抑制VSMCs迁移（P〈0．01），但三个抑制剂组之间差异无统计学意义（P〉0．05）；划痕后30h，EGF组VSMCs迁移仍较对照组明显（P〈0．01），WT组、SB组及PD组与EGF组相比则明显抑制VSMCs迁移（P〈0．叭），且三个抑制剂组之间差异有统计学意义，其中sB组较WT组和PD组抑制VSMCs的迁移更明显（P〈0．01）。结论EGF诱导VSMCs迁移可能通过P13K、P38MAPK及ERK1／2途径起作用，且P38MAPK途径作用更明显。Objective To study the effects of Wortmannin, inhibitor of PI3K and SB202190, inhibitor of P38 MAPK and PD98059, inhibitor of ERK1/2 on the migration of epidermal growth factor （EGF）-induced vascular smooth muscle cells （VSMCs）. Methods There were fives groups in this experiment, including control group, EGF group, PD98059 （PD） group, SB202190 （SB） group and Wortmannin （WT） group. The migration rate of the VSMCs was measured by wound healing assay. Results At the 24th hours after wounding, there was obvious migration in EGF group compared to control group. The migration of VSMCs was significantly inhibited in PD group, SB group and WT group compared to the EGF group, but there were no significant difference among three inhibitor groups. At the 30th hours after wounding, there was still obvious migration in EGF group compared to control group. The migration of VSMCs was significantly inhibited in the three inhibitors group compared to the EGF group, and there were significant difference among three inhibitor groups. Furthermore, inhibiting effect on VSMCs in SB group was more obvious compared to PD group and WT group. Conclusion These results suggested that the migration of EGF-induced VSMCs may play a role through PI3K, P38 MAPK and ERK1/2 signal pathways, and the effect of P38 MAPK signal pathway is very important.

关 键 词：1-磷脂酰肌醇3-激酶/拮抗剂和抑制剂 p38丝裂原活化蛋白激酶类/拮抗剂和抑制剂 肌细胞 平滑肌/药物作用 表皮生长因子 

分 类 号：R285.5[医药卫生—中药学]