蛋白激酶B(AKT)抑制剂对小鼠1-细胞期胚胎分裂的影响  

Effect of Protein Kinase B (AKT) Inhibitors on Cell Division of Mouse 1-Cell Embryos

作　　者：邓欣[1] 李亘松[1] 朱丽娜[1] 周洪伟[1] 宋阳[1] 刘莹[2] 崔城[3] 孙小涵[2] 于秉治[2] 

机构地区：[1]中国医科大学机能实验中心,沈阳110001 [2]中国医科大学生物化学与分子生物学教研室,沈阳110001 [3]中国医科大学生理教研室,沈阳110001

出　　处：《生殖与避孕》2009年第6期349-353,389,共6页Reproduction and Contraception

基　　金：辽宁省科技厅博士启动基金(20041043);辽宁省高校学校科研项目(2008S224)

摘　　要：目的:研究蛋白激酶B(AKT)在小鼠1-细胞期受精卵中的作用。方法:激酶活性分析检测2种AKT的抑制剂在小鼠1-细胞期胚胎中对MPF活性的调节;G2/M期的细胞计数检测AKT抑制剂对小鼠1-细胞期胚胎细胞分裂的影响;Western blotting检测AKT抑制剂对小鼠1-细胞期胚胎中pCDC2Tyr15的去磷酸化状态的改变。结果:AKT抑制剂抑制了MPF活性、G2/M期的转换,pCDC2Tyr15的去磷酸化状态。结论:AKT存在于小鼠1-细胞期胚胎中并通过调节MPF活性和pCDC2Tyr15的去磷酸化状态调节细胞分裂的进程。Objective： To study the effect of AKT cell division of mouse 1-cell embryos. Methods： Effect of AKT inhibitors on MPF activity, cell division and dephosphorylation status of pCDC2 Tyrl5 in mouse 1-cell embryos was detected respectively by activity analysis, counting under dissecting microscope and Western blotting. Results： MPF activity was blocked by the inhibitors of AKT, division rate of 1-cell embryos was decreased by the inhibitors of AKT, dephosphorylation status of pCDC2 Tyr 15 was blocked by the inhibitors of AKT. Conclusion： In 1-cell embryos of mouse, MPF activity, cell division and pCDC2 tyr 15 were regulated by AKT inhibitors.

关 键 词：蛋白激酶B(AKT) 小鼠 受精卵 G2/M转换 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]