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作 者:朱文奇[1] 李慧芳[1] 宋卫涛[1] 吴蓉蓉[2] 徐文娟[1] 于吉英 肖小君[2,3] 陈宽维[1]
机构地区:[1]中国农业科学院家禽研究所,江苏扬州225003 [2]扬州大学动物科学与技术学院,江苏扬州225003 [3]海南罗牛山文昌鸡育种有限公司,海南海口570000
出 处:《安徽农业科学》2009年第18期8357-8358,共2页Journal of Anhui Agricultural Sciences
基 金:国家科技支撑计划(2008BADB2B07);国家高技术研究发展计划(2006AA10Z1D8);农业行业专项研究计划(nyhyzx07-039);现代农业产业技术体系建设专项资金
摘 要:[目的]检测文昌鸡神经肽Y基因(NPY)的多态性及其与开产性状的关系,为高产文昌鸡的育种提供资料。[方法]通过PCR-RFLP方法检测文昌鸡NPY基因DraⅠ位点上的多态性,分析其基因型、群体的遗传稳定性以及该位点多态性与开产日龄、开产体重和开产蛋重的关系。[结果]文昌鸡在NPY基因DraⅠ位点上有3种基因型(AA、AB、BB);基因型的分布符合Hardy-Weinberg定律;不同基因型间的开产日龄、开产蛋重差异不显著(P>0.05),开产体重差异极显著(P<0.01)。[结论]文昌鸡NPY基因DraⅠ位点处于Hardy-Weinberg平衡状态,其基因多态影响开产体重。[Objective] The paper presents the polymorphism of neuropeptide Y gene (NPY) and its relationship with traits at first laying in Wenchang chicken, in order to provide information for breeding of high-yielding Wenchang chicken. [Method] The polymorphism of NPY gene at Dra I site was detected by PCR-RFLP, and the genotypes and the genetic stability were determined. The correlation between the polymorphism and traits at first laying (age, body weight, and egg weight) was also analyzed. [ Result] There were three kinds of genotype of NPY gene at Dra I site in Wenchang chicken, designated as AA, AB, and BB ; the distribution of these genotypes was consistent with the Hardy - Weinherg law; age and egg weight were not significantly different among the three genotypes (P 〉0.05), and body weight was extremely significantly different (P〈0.01). [ Conclusion ] The NPY gene of Wenchang chicken is in Hardy - Weinberg equilibrium at Dra I site, and its genetic polymorphismaffects body weight at first laying.
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