“大通牦牛”Lfcin基因克隆及生物信息学分析  

Clone and Bioinformatic Analysis of Lfcin Gene of Datong Yak

作　　者：裴杰[1] 阎萍[1] 冯瑞林[1] 梁春年[1] 郭宪[1] 曾玉峰[1] 包鹏甲[1] 朱新书[1] 褚敏[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050

出　　处：《安徽农业科学》2009年第18期8380-8382,8395,共4页Journal of Anhui Agricultural Sciences

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(BRF070105)

摘　　要：[目的]克隆"大通牦牛"Lfcin基因,为将该基因应用于饲料工业和养殖业提供依据。[方法]利用PCR技术从"大通牦牛"基因组DNA中获得乳铁蛋白素(Lactoferricin,Lfcin)基因序列;将Lfcin基因连接于pGEM-T easy载体,送至生物公司测序;将"大通牦牛"与奶牛的Lfcin基因序列进行比对;同时,对牦牛、奶牛、人、小鼠等物种的Lfcin蛋白进行序列分析和进化树分析。[结果]克隆获得了含"大通牦牛"LF(Lactoferrin)第二外显子的DNA序列,共778 bp,其中Lfcin基因编码区长75 bp,编码25个氨基酸;序列分析显示,克隆获得的牦牛DNA序列与奶牛该序列存在11个碱基的变异;牦牛和奶牛的Lfcin蛋白质序列完全相同,各物种Lfcin蛋白具有较高的同源性;进化树分析表明Lfcin进化树符合物种进化规律。[结论]该研究为Lfcin基因在原核或真核细胞中的表达研究以及进一步研究Lfcin蛋白的生活活性奠定了基础。[Objective] The experiment aimed to provide reference for using this gene into feed industry and breeding industry by cloning Lfcin gene of Datong Yak. [Method] PCR method was used to obtain gene sequence of Lactoferricin （Lfcin） from genomic DNA of Datong Yak. The Lfcin gene was connected to pGEM-T easy vector then it was sequenced by Biology Company. The Lactoferricin （Lfcin） gene of Datong Yak was compared with that of cow. Meanwhile, Lfcin genes of yak, cow, human and mouse were conducted sequence analysis and phylogenetic analysis.[Result] Through clone, the DNA sequence of Datong Yak containing exon 2 was obtained and it was 778 bp. Among them, the coding region of Lfcin gene was 75 bp and it encoded 25 amino acids. It was concluded from sequence analysis that there were llvariant bases in DNA sequences of yak and cow while the Lfcin protein sequences of yak and cow were same, so Lfcin protein of all species had high homology. The phylogenetic analysis showed that Lfcin phylogenetic tree accorded with species evolution laws. [Conclusion] The research laid foun- dations for expression study of Lfcin gene in prokaryotic cells and eukaryotic ceils as well as further study of biological activity of Lfcin protein.

关 键 词：牦牛 Lfcin基因 克隆 生物信息学 

分 类 号：S823[农业科学—畜牧学]