极度濒危植物百山祖冷杉水培繁殖  

Propagation of Abies beshanzuensis by Water Cultured Medium

作　　者：饶龙兵[1] 吴友贵[2] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [2]百山祖自然保护区,浙江庆元323800

出　　处：《安徽农业科学》2009年第18期8477-8478,共2页Journal of Anhui Agricultural Sciences

基　　金：浙江省科技计划项目(2005C32036);国家自然科学基金项目(30700644)

摘　　要：[目的]研究植物外源激素(ABT和IAA)和营养液对百山祖冷杉水培生根的作用。[方法]设清水不加任何激素(CK)、清水+10mg/L ABT、清水+10 mg/L IAA、10 mg/L ABT+霍格兰营养液、10 mg/L IAA+霍格兰营养液5个处理进行水培百山祖冷杉,观察插条生根情况,统计愈伤形成率、生根率、生根数、根长等。[结果]ABT和IAA对百山祖冷杉水培愈伤诱导和生根率、生根数有明显促进作用,两者都适合在百山祖冷杉水培繁殖中应用;外源激素处理对最长根长和平均根长无规律性影响;有无营养液处理对百山祖冷杉水培繁殖前期各项均无影响,但在出根后会影响根的生长。[结论]研究为百山祖冷杉繁育提供了新思路,有望使其迅速脱离濒危现状。[Objective] The experiment aimed to explore the influences of phytohormones （ABT and IAA） and nutrient solution on rooting of Abies beshanzuensis M. H. Wu by water cultured medium.[Method] The Abies beshanzuensis M. H. Wu were treated by water （CK） , water ＋ 10mg/L ABT, water ＋ 10 mg/L IAA, 10 mg/L ABT ＋ hoagland solution, 10 mg/L IAA ＋ hoagland solution, then the rooting process was observed and the formation rate of callus, rooting rate, number of rooting, and root length were observed. [Result] ABT and IAA had obvious influences on callus induction of Abies beshanzuensis M. H. Wu by water culture, rooting rate, the number of root, so they were suitable to be used in water propagation of Abies beshanzuensis M. H. Wu. The treatments of phytohormones had no regular influences on longest root length and average root length. The nutrient solutions would not generate obvious influence on propagation of Abies beshanzuensis M. H. Wu at firstly stage, but they generated influence on root growth after rooting.[Conclusion] The research provided new ideas for propagation of Abies beshanzuensis M. H. Wu, which could make it out of endangerment situation quickly.

关 键 词：百山祖冷杉 繁殖 水培 植物激素 营养液 

分 类 号：S723.132[农业科学—林木遗传育种]