长丝鲈5种组织中相关酶的测定  

Determination on Relative Enzymes in 5 Kinds of Organizations of Osphronemas goramy

作　　者：魏东[1] 董少杰[1] 孙向军[2] 梁拥军[2] 穆祥兆[2] 

机构地区：[1]天津农学院水产科学系/天津市水产生态及养殖重点实验室,天津300384 [2]北京市水产科学研究所,北京100068

出　　处：《安徽农业科学》2009年第18期8535-8537,8540,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]测定了长丝鲈5种组织中与免疫学和病理学相关酶的活性。[方法]以购自小汤山水产养殖基地的17尾健康长丝鲈为试材，鲈鱼体重194.42-295.74g，体长19.24-21.10cm，检测了长丝鲈脑、肝、肌肉、肠和鳃5种组织中的谷胱甘肽硫转移酶（GST）、过氧化氢酶（CAT）、转氨酶（GOT、GPT）、乳酸脱氢酶（LDH）和磷酸酶（ACP、AKP）的活性以及脂质氧化产物（MDA）的含量。[结果]长丝鲈脑组织中7种酶的活性大小为LDH〉GST〉GPT〉GOT〉CAT〉AKP〉ACP；肝组织LDH〉CAT〉GST〉GPT〉GOT〉AKP〉ACP；肌肉组织LDH〉GST〉GPT〉GOT〉CAT〉AKP〉ACP；肠组织LDH〉GST〉AKP〉ACP〉GPT〉GOT〉CAT；鳃组织LDH〉GST〉AKP〉ACP〉CAT〉GPT〉GOT。长丝鲈5种组织中，丙二醛的含量高低依次为鳃〉脑〉肠〉肌肉〉肝。[结论]该研究为长丝鲈免疫学和病理学等方面的研究提供基础理论数据。[Objective] The study aimed to determine the activities of enzymes relative to immunology and pathology in 5 kinds of organizations of Osphronemas goramy. [Method] With 17 tails of health O.goramy, body weight of 194.42-295.74 g and body length of 19.24 -21.10 cm, purchased from Xiaotangshan aquaculture base as the tested materials, the activities of 7 kinds of enzymes （GST, CAT, GOT, GPT, LDH, ACP and AKP） and the MDA content in 5 kinds of tissues （brain, liver, muscle, intestine and lamella） of O. goramy were detected. [Result] The activities of 7 kinds of enzymes in brain tissue of O. goramy in order were LDH 〉 GST 〉 GPT 〉 GOT 〉 CAT 〉AKP 〉 ACP, that in liver tissue wereLDH 〉 CAT 〉 GST 〉 GPT 〉 GOT 〉 AKP 〉 ACP, that in muscle tissue were LDH 〉 GST 〉 GPT 〉 GOT 〉 CAT 〉 AKP 〉 ACP, that in intestine tissue were LDH 〉 GST 〉 AKP 〉 ACP 〉 GPT 〉 GOT 〉 CAT and that in lamella tissue were LDH 〉 GST 〉 AKP 〉 ACP 〉 CAT 〉 GPT 〉 GOT. The content of MDA in 5 kinds of tissues of O. goramy in order was lamella 〉 brain 〉 intestine 〉 muscle 〉 liver. [Conclusion] The study provided the basic theoretical data for the research of O. goramy on immunology and pathology.

关 键 词：长丝鲈 酶 活性 丙二醛 

分 类 号：S917[农业科学—水产科学]