双色荧光原位杂交检测男性不育患者精子X和Y染色体  

The Establishment of an Approach to Dual-color Fluorescence in Situ Hybridization of Sperm Chromosome X and Y in Male Infertility

作　　者：莫毅 谢丹尼 檀大羡 陈美佳 陈自洪 吕福通 陈一君 

机构地区：[1]广西人口和计划生育研究中心,南宁市530021

出　　处：《广西医学》2009年第6期761-763,共3页Guangxi Medical Journal

基　　金：广西壮族自治区人口和计划生育委员会科研课题(桂计生研0103)

摘　　要：目的建立男性不育患者精子核双色荧光原位杂交技术(FISH)的检测方法。方法将20例男性不育患者精子标本经低渗液处理、固定后制片,用二硫苏糖醇(DTT)使精子去凝集,变性后与双色荧光标记(CEP X、Y)探针杂交。结果20例患者的6 672个精子中,X染色体精子率为47.78%,Y染色体精子率为47.24%,性染色体数目异常的精子率为4.53%,有效杂交率为99.55%。结论成功地建立了检测人精子X和Y染色体的检测方法。Objective We aim to establish an approach to dual-color fluorescence in situ hybridization （FISH） of sperm chromosome X and Y in male infertility. Methods Twenty cases＇samples were collected,later washed with 1 x PBS and treated with KCl hypotonic solution, then immobilized with methanol and acetic acid （3 ： 1 ） ,dropped on the slides and agglutinated with DTT. Finally,the dual-color FISH were performed on human sperm nucleus using centromeric probes for chromosome X and Y. Results There were 6 672 sperms were counted in this experiment,X chromosome rate Was 47.78%, Y chromosome rate was 47.24%, sex chromosome abnormal rate was 4.53%, and hybridization rate was 99.55%. Conclusion Dual-color FISH method to analyze sperm chromosome X and Y in male infertility had been set up successfully.

关 键 词：荧光原位杂交技术 不育症 精子 染色体 男性 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学] Q32[医药卫生—基础医学]