纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的实验研究  

Experimental study on effect of titanium fiber mesh microballoons combined with Nano-Sr-HAP on repairing rat bone defects

作　　者：王楠[1] 郝永强[1] 何国[2] 朱邦尚[3] 金芳纯[1] 于晓巍[1] 刘萍[2] 倪嘉桦[2] 

机构地区：[1]上海交通大学附属第九人民医院骨科,200011 [2]上海交通大学材料科学与工程学院 [3]上海交通大学分析测试中心

出　　处：《中华骨科杂志》2009年第7期672-676,共5页Chinese Journal of Orthopaedics

基　　金：上海市骨科内植物重点实验室建设基金（08DZ2230330）;上海市卫生局局级课题（2008124）

摘　　要：目的探讨纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的能力及其作用机制。方法6月龄雄性SD大鼠24只，体重（545±22）g。在双删股骨髁部使用慢速钻钻取直径2mm贯通双侧皮质的冠状轴洞性骨缺损。以纤维多孔钛微球复合纳米锶磷灰石填充左侧骨缺损，以单纯纤维多孔钛微球填充右侧。术后1、2、4、8周分别处死6只大鼠行X线、组织学及骨组织形态计量学观察，并进行比较分析。结果影像学结果表明，两侧骨缺损修复效果均良好。组织学显示，纤维多孔钛微球允许骨长入，且左侧微球内新生骨多于右侧。骨组织形态计量学观察显示，随时间延长，左侧新生骨量逐渐增多，2、4、8周新生骨量存在差异，而4、8周时左侧与右侧的新生骨量比较，差异有统计学意义。结论纤维多孔钛微球具有良好的生物相容性及骨传导性，可作为骨缺损修复的支架材料；纳米锶磷灰石可增强纤维多孑L钛微球修复骨缺损的能力。Objective To evaluate the effect of titanium fiber mesh microballoons combined with nano-Sr-HAP on repair of bone defects in vivo. Methods The experimental model was set up by creating drill defects in the bilateral femoral condyle of 24 SD rats [6 months, with the mean weight of （545±22 g）]. The left femoral defects were filled with titanium fiber mesh mieroballoons combined with nano-Sr-HAP, and the fight with pure metal scaffolds. At the 1st, 2nd, 4th, and 8th week after surgery, the specimens were killed and taken the X-ray, histology, and histomorphology observation. The results were contrasted and ana- lyzed. Results X-ray observation showed that the bone defects in both sides were repaired. Histological resuits showed that the titanium fiber mesh microballoons allowed the new bones growing inside, and more new bones in the left implants compared with those in the right sides. The results of histomorphometrical analysis confirmed that neo-bones in the left sides gradually increased as time passed. There were statistic differences in neo-bones at the 2nd, 4th, and 8th week, and the differences between the two sides were statistically significant at the 4th and 8th week. Conclusion As titanium fiber mesh mieroballoons used in this experiment have good biocompatibility and osteoconduction ability, they can be used as scaffold for bone defect repair; and Nano-Sr-HAP can enhance the repair ability of titanium fiber mesh.

关 键 词：钛 微球体 纳米技术 锶 磷灰石 

分 类 号：R686[医药卫生—骨科学]