激发-发射矩阵荧光结合二阶校正法测定人体血浆样和牛蒡子药材中的牛蒡苷含量  

Determination of arctiin in plasma samples and arctium lappa L.by using excitation-emission matrix fluorescence and second-order calibration based on parallel factor analysis/alternating normalization-weighted error algorithms

作　　者：张燕[1] 吴海龙[1] 欧阳立群[1] 万瑶[1] 俞汝勤[1] 

机构地区：[1]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《计算机与应用化学》2009年第6期697-704,共8页Computers and Applied Chemistry

基　　金：国家自然科学基金项目(20775025);973预研课题(2007CB216404)

摘　　要：本文利用激发-发射矩阵荧光与二阶校正算法相结合,实现快速测定人体血浆样和牛蒡子对照药材中的牛蒡苷含量。采用二阶校正方法的平行因子分析(PARAFAC)和交替归一加权残差(ANWE)两种算法进行解析,所得血浆基体干扰下牛蒡苷的回收率分别为(98.7±1.4)%和(99.7±1.6)%,实现了对血浆样中的牛蒡苷含量的直接快速定量测定。此外,用上述两种方法对牛蒡子药材中的牛蒡苷含量进行快速测定,所得结果与液相色谱方法结果进行比较,结果令人满意。实验结果表明,此方法方便快捷,可用于复杂试样中未知干扰共存下牛蒡苷含量的测定。This paper proposed a new approach for direct determination of arctiin in plasma samples as well as a Chinese medicine （ Arctium lappa L. ） by the combination of excitation-emission matrix fluorescence and second-order calibration methods based on parallel factor analysis（PARAFAC） and alternating normalization-weighted error（ANWE） algorithms. The average recoveries of actiin in plasma samples obtained by using PARAFAC and ANWE with the estimated component number of two were 98.7 ± 1.4% and 99. 7 ± 1.6%, respectively. The methodology was used to estimate the content of arctiin in Arctium lappa L. , and the obtained results were also satisfactory compared with that of the HPLC method. It can be seen that the suggested methods can be emp!oyed to directly deter- mine arctiin, even in the presence of unknown and un-calibrated interferences, only with a simple pretreatment procedure.

关 键 词：牛蒡苷 血浆 牛蒡子药材 三维荧光光谱 二阶校正 平行因子分析(PARAFAC) 交替归一加权残差 (ANWE) 

分 类 号：O6-39[理学—化学] TQ015.9[化学工程]