蝙蝠葛酚性碱对BXPC-3荷瘤抑制作用及iNOS含量的影响研究  

Inhibitory Effect of Phenolicalkaloids of Menispermum Dauricum on the BXPC-3 Tumor and Its Effect on iNOS Content in Tumor-bearing Mice

作　　者：闻喜英[1] 焦广宇[2] 李卉[3] 苏云明[3] 

机构地区：[1]牡丹江医学院第二附属医院药剂科,牡丹江市157009 [2]哈尔滨医科大学,哈尔滨市150000 [3]黑龙江中医药大学,哈尔滨市150000

出　　处：《中国药房》2009年第18期1370-1372,共3页China Pharmacy

基　　金：黑龙江省自然科学基金项目(D200675)

摘　　要：目的:研究蝙蝠葛酚性碱(PAMD)对人胰腺癌细胞株(BXPC-3)荷瘤的抑制作用及外周血诱生型一氧化氮合酶(iNOS)含量的影响。方法:复制BXPC-3荷瘤裸鼠模型,复制模型后裸鼠随机均分为6组,即模型对照、空白对照、环磷酰胺及PAMD高、中、低剂量组;空白对照组不接种瘤株,给予同体积的生理盐水。检测PAMD对肿瘤的抑制率;采用分光光度法检测荷瘤裸鼠外周血iNOS含量变化。结果:PAMD高、中、低剂量对BXPC-3荷瘤裸鼠均有显著抑制肿瘤生长的作用,其抑瘤率分别为34.91%、52.83%、41.51%;与模型对照组比较,PAMD药物组BXPC-3荷瘤裸鼠血清iNOS的含量显著降低。结论:PAMD对BXPC-3荷瘤裸鼠具有显著抑瘤作用,其抑瘤作用可能与通过降低血清iNOS含量有关。OBJECTIVE： To observe the inhibitory effect of phenoliealkaloids of menispermum dauricum （PAMD） on the human pancreatic cell line（BXPC- 3） tumor and study the effect of PAMD on serum content of inducible nitric oxide synthase （iNOS） in tumor-bearing mice. METHODS： The BXPC- 3 tumor- bearing mice model was established, and the model mice were randomly assigned to 6 groups： model control group, blank control group, cyclophosphamide group, 3 PAMD groups （high, medium and low dosages） . The blank control group was not inoculated with tumor strain but given same volume of normal saline. The tumor- inhibition ratio of PAMD was detected and the content of iNOS of in peripheral blood of tumor- bearing mice was determined by fluorospectrophotometry. RESULTS： PAMD （high, medium and low dosages） showed marked inhibitory effect on tumor growth of BXPC-3 cell line in tumor-bearing mice, with the inhibition ratios at 34.91%, 52.83% and 41.51%, respectively. As compared with model control group, PAMD-treated groups showed significantly lower content of iNOS in peripheral blood of tumor - bearing mice. CONCLUSION： PAMD showed marked inhibitory effect on tu- mor growth of BXPC- 3 cell line in tumor- bearing mice, which might be attributed to the lowered content of iNOS.

关 键 词：蝙蝠葛酚性碱 诱生型一氧化氮合酶 人胰腺癌细胞株 

分 类 号：R285[医药卫生—中药学] R932[医药卫生—中医学]