紫外分光光度法测定马鞭草中微量元素硒的含量  

Determination of Trace Element Selenium in Verbena officinalis by Ultraviolet Spectrophotometry

作　　者：卞杰松[1] 邓斌[1] 黄红英[1] 章爱华[2] 刘志伟 

机构地区：[1]湘南学院化学与生命科学系,郴州市423000 [2]吉首大学化学化工学院,吉首市416000 [3]浙江海正药业股份有限公司,台州市318000

出　　处：《中国药房》2009年第18期1401-1403,共3页China Pharmacy

基　　金：2007年湖南省自然科学基金计划项目(07JJ3017)

摘　　要：目的:测定马鞭草中微量元素硒的含量。方法:采用紫外分光光度法,以邻苯二胺盐作显色剂,检测波长为335nm。结果:硒的检测浓度在0～0.10μg.mL-1范围内与吸光度呈良好的线性关系(r=0.9993);平均回收率为99.78%,RSD=0.29%(n=9)。结论:本方法简便、灵敏、准确,可用于马鞭草中微量元素硒的含量测定。OBJECTIVE： To determine the content of selenium in Verbena officinalis .METHODS： The content of selenium in Verbena officinalis was determined by UV spectrophotometry with o- phenylenediamine as developer under a detection wavelength of 335 nm. RESULTS： The linear range of selenium was 0-0.10μg·mL^-1（r=0. 999 3） with an average recovery of 99.78% （RSD = 0.29%, n = 9） ~ CONCLUSION： The method was proved to be simple, sensitive and accurate, and applicable for the determination of trace element selenium in Verbena officinalis.

关 键 词：马鞭草 硒 含量 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]