葡萄籽提取物对皮肤急性光损伤基质金属蛋白酶-1表达的影响  

Influence of grape seed proanthocyanidin extract on MMP-1 expression in acute ultraviolet injury

作　　者：袁小英[1] 刘玮[2] 江丽[1] 张洁[1] 

机构地区：[1]中国人民解放军空军总医院皮肤科,北京100036 [2]中国医科大学

出　　处：《中国麻风皮肤病杂志》2009年第6期407-409,共3页China Journal of Leprosy and Skin Diseases

基　　金：本课题受资生堂(中国)研究开发有限公司赞助;项目为中医健康.美容

摘　　要：目的:评价外用葡萄籽提取物原花青素(grape seed proanthocyanidin extract,GSPE)对急性光损伤的基质金属蛋白酶-1(MMP-1)表达的影响。方法:实验没计空白对照组、单纯2 MED照射组、基质+2 MED照射组、含GSPE样品外用+2 MED照射组4绀,连续3天同样处理后皮肤取材,进行MMP-1免疫组化染色。结果:含GSPE外用+2 MED照射组与单纯接受紫外线照射的皮肤相比,MMP-1表达明显减少,MMP-1蛋白积分明显下降,差异有显著性(P<0.01)。结论:GSPE外用可通过降低MMP-1的表达对紫外线急性光损伤起防护作用。Objective： To assess the influence of grape seed proanthocyanidin extract （GSPE） on MMP- 1 expression inacute ultraviolet injury. Methods： MMP- 1 expression was measured by MMP- 1 immunohistochemical method in skin samples taken from normal skin, skin treated with 2 MED UV radiation, skin treated with vehicle plus 2 MED UV radiation and skin treated with GPSE solution plus 2 MED UV radiation for 30 minutes. Results： MMP- 1 expression and the MMP- 1 protein score is significantly decreased in the samples treated with GSPE plus 2 MED UV radiation, compared with skin treated with 2 MED UV radiation only （P 〈 0.01 respectively）. Conclusions： GSPE may protect skin from UV radiation by reduction of the expression of MMP- 1.

关 键 词：葡萄籽提取物原花青素 基质金属蛋白酶-1 临床分析 治疗方法 

分 类 号：R285.5[医药卫生—中药学]