RP-HPLC法测定不同品种不同产地柴胡中柴胡皂苷a、d的含量  

Determination of Saikosaponin a,d of Radix Bupleuri from Different Species and Areas in China by RP-HPLC

作　　者：白杨[1] 胡继鹰[2] 

机构地区：[1]广州军区武汉总医院临床药理科,武汉430070 [2]湖北中医学院基础医学院

出　　处：《中国药师》2009年第7期852-854,共3页China Pharmacist

基　　金：湖北省自然科学基金(2005ABA187)

摘　　要：目的:建立柴胡中柴胡皂苷a、d含量的RP-HPLC测定方法,以分别考察不同种植基地柴胡及野生柴胡中柴胡皂苷a、d的含量。方法:采用BonChrom C_(18)色谱柱(200mm×4.6 nm,5μm),流动相为乙腈-水(40∶60),流速1.0ml·min^(-1),检测波长204nm。结果:柴胡皂苷a线性范围为1.94～19.44μg(r=0.999 9),柴胡皂苷d线性范围为1.96～19.60μg(r=0.999 8),柴胡皂苷a平均回收率为99.35%(RSD=1.14%),柴胡皂苷d平均回收率为99.52%(RSD=1.18%)。不同品种不同产地柴胡中柴胡皂苷a、d含量之和的分布范围为0.44%～0.67%。结论:本法简便快捷,结果准确,重复性好,地道中药材规范化种植(GAP)基地的柴胡皂苷含量较高。Objective： To establish an optimal RP-HPLC method to determine the content of saikosaponin a, d of Radix Bupleuri from different species or areas in China. Method： Saikosaponin a,d were separated on BonChrom ODS C18 eolumn（200mm ×4.6 mm, 5μm）with aeetonitrile-water（40： 60） as the mobile phase and with a flow rate of 1.0 ml ·min^-1. The detecting wavelength was at 204nm. Result： The linear ranges of salkosaponin a and saikosaponin d were 1.94 - 19. 44μg （ r = 0. 999 9 ） and 1.96 - 19.60 μg（ r = 0.999 8）, and the average recoveries of them were 99.35% （RSD = 1.14% ） and 99.52% （RSD = 1.18% ）, respectively. The contents of saikasaponin a and saikosaponin d of Radix Bupleuri in different species or areas were 0.44% -0.67%. Conclusion： This method is simple, rapid, accurate and reproducible. Three GAP base areas of Bupleurum chinense have higher Saikosaponin contents.

关 键 词：柴胡 柴胡皂苷A d 反相高效液相色谱 含量测定 

分 类 号：R927.2[医药卫生—药学]