中药提取物逆转K562／ADM细胞的耐药作用及其分子机制的探讨  

Reversal of multidrug resistance of K562/ADM cells in vitro by the monomer of traditional Chinese medicine extract and investigation of its molecular mechanism

作　　者：乔高娟[1] 李贵海[2] 杨炜华[1] 温培娥[1] 任霞[1] 范华[1] 唐天华[1] 任海全[1] 姜国胜[1] 

机构地区：[1]山东省医学科学院基础医学研究所山东省医药卫生肿瘤免疫与中药免疫重点实验室,山东济南250062 [2]山东省中医药研究院

出　　处：《国际肿瘤学杂志》2009年第6期474-476,共3页Journal of International Oncology

基　　金：山东省自然科学基金资助项目（Y2005C57）;山东省科技项目（2008GG2NS02023）.

摘　　要：目的观察两种中药提取物黄芩苷、京尼平苷对多药耐药（MDR）K562／ADM细胞的耐药逆转作用，探讨其分子机制。方法建立阿霉素（ADM）诱导K562／ADM细胞耐药模型，分别将不同浓度黄芩苷、京尼平苷作用于K562／ADM细胞，用四甲基偶氮唑盐（MTT）法检测其对化疗药物的敏感性，用逆转录聚合酶链反应（RT-PCR）检测多药耐药基因-1（mdr-1）、拓扑异构酶Ⅱ（TopoⅡ）基因在mRNA水平的表达变化。结果两种中药提取物黄芩苷、京尼平苷，可增加K562／ADM细胞对ADM的敏感性，K562／ADM细胞增殖受到明显抑制，耐药逆转倍数分别为1.95倍及1.46倍；RT-PCR检测mdr-1基因表达减弱，TopoⅡ基因表达增强（P〈0.01）。结论黄芩苷、京尼平苷可通过降低K562／ADM细胞mdr-1基因表达和提高TopoⅡ的表达来逆转白血病细胞MDR。Objective To investigate the reversal effect of the monomer of traditional Chinese medicine on muhidrug resistance（MDR） and its possible mechanism in K562/ADM cell line in vitro. Methods With different concentrations of baicalin, geniposide administered to K562/ADM cells, the proliferation of K562/ ADM cells was detected by the MTT assay. Expression of mdr-1 mRNA, Topo Ⅱ mRNA was measured by semiquantitive RT-PCR. Results Thatbaicalin and geniposide could increase the sensitivity of K562/ADM cells to adriamycin, multiples of reversion were 1.95 times and 1.46 times. The proliferation of K562/ADM cells was inhibited obviously by baicalin and geniposide, the level of mdr-1 mRNA expression was down-regulated and the Topo Ⅱ mRNA was up-regulated （P〈0.01）. Conclusion Baicalin and geniposide may reverse the multi-drug-resistance of K562/ADM cells, which was related to the down-regulation of mdr-1 expression and up-regulation of Topo Ⅱ beta expression.

关 键 词：黄芩 栀子 P糖蛋白 白血病 

分 类 号：R285.5[医药卫生—中药学]