负载吡啶盐染料二氧化硅纳米粒子的制备及其光学性能  

Preparation and optical property of fluorescent silica nanoparticles entrapping pyridinium dyes

作　　者：周盛蕊[1] 王明亮[1] 徐春祥[2] 吴伟兵[1] 崔一平[2] 刘畅[2] 黄维[2] 

机构地区：[1]东南大学化学化工学院,南京210096 [2]东南大学电子科学与工程学院,南京210096

出　　处：《东南大学学报（自然科学版）》2009年第3期606-609,共4页Journal of Southeast University：Natural Science Edition

基　　金：江苏省高技术研究计划资助项目(BG2006006);国家高技术研究发展计划(863计划)资助项目(2006AA03Z313);国家自然科学基金资助项目(60725413;60576008;10674023);国家重点基础研究发展计划(973计划)资助项目(2007CB936300)

摘　　要：采用反相微乳液法制备了负载2种吡啶盐染料的二氧化硅荧光纳米粒子.制备出的纳米粒子呈单分散球形,平均粒径为30nm.通过研究其在氯仿、乙醇、N,N-二甲基甲酰胺(DMF)、二甲基亚砜(DMSO)和水中的紫外及荧光性质,发现吡啶盐染料负载于二氧化硅纳米粒子后,克服了吡啶盐的荧光最大发射波长随着溶剂极性而变化的现象,有效地抑制了吡啶盐在高浓度下的浓度淬灭效应,并提高了材料的抗光漂白性能.在254nm激发光照射25h后,荧光纳米粒子分散液在460nm处的吸光度是原来的68%,而染料溶液只有原来的11%.研究表明,这类纳米粒子作为一种新型的荧光纳米探针可用作高灵敏度的生物检测及细胞成像.Fluorescent silica nanoparticles entrapped in two different pyridinium dyes were prepared by reverse microemulsion method. The nanoparticles are mono-dispersed, spherical and the particle average size is 30 nm. Their ultraviolet and fluorescent properties in chloroform, ethanol, N, N- Dimethylformamide（DMF）, dimethyl sulfoxide（DMSO） and water were investigated. It is fouud that the fluorescence of the nanoparticles is not influenced by solvent polarity and a concentration quenching effect is inhibited; the anti-photobleaching capability is also enhanced. After 25 h irradiation of 254 nm excited light, the nanoparticle dispersion＇s absorbency remains at 68% in the wavelength of 460 nm; however, for the pyridinium dye solution it is only 11% under the same conditions. It implies that these silica nanoparticles can be used as a new kind of nano-probe in highlysensitive biological detection and biological imaging.

关 键 词：吡啶盐 二氧化硅纳米粒子 制备 光学性能 

分 类 号：TQ127.2[化学工程—无机化工]