低钾胁迫下两种不同耐低钾大豆叶片显微结构的差异  

Differences of Low Potassium Stress on Leaves Microstructure of Two Soybean Varieties with Different Resistance

作　　者：曲婷婷[1] 曹敏建[1] 李兴涛[1] 王伟[1] 

机构地区：[1]沈阳农业大学农学院,辽宁沈阳110161

出　　处：《大豆科学》2009年第3期434-438,共5页Soybean Science

摘　　要：以耐低钾大豆品种九农15和不耐低钾品系GD8521为试材,用水培方法研究了低钾胁迫下两种类型大豆叶片显微结构的差异。结果表明:在低钾胁迫下,耐性品种的上表皮结构变化不明显;而不耐品种的上表皮厚度增加,上表皮细胞长度、上表皮细胞密度及上表皮长/宽都减小;低钾胁迫下,耐性品种的栅栏组织仅第一层细胞长度减小,而不耐品种第一、二层栅栏组织细胞长度、宽度均增加,细胞密度减少,栅栏组织增厚;随着供钾水平的降低,耐性品种仍保持较发达的平脉叶肉细胞,而不耐品种平脉叶肉细胞不发达;低钾胁迫下耐性品种海绵组织细胞密度减少,不耐品种栅海比变化显著增大;低钾胁迫下,耐性品种下表皮略有增厚,不耐品种几乎没有差异。In former thesis, difference of photosynthetic ability due to low K stress between two kinds of resistant varieties had been reported in soybean, but the change of leaves microstructure and factors led to this difference still unclear. So in this experiment, we selected two soybean varieties（lines） with different resistance as material in order to study the effect of low potassium on microstructure of soybean leaves, so as to probing into tolerant mechanism of different resistant varieties on low potassium stress. The plants were grown in modified 1/2Hoaglands＇ solution with 0.5 mmol. L^-1 （low potassium stress） and 3.0 mmol.L^- 1 of K. Results showed there was no difference in upper epidermis microstructure between low potassium stress and CK for tolerant variety. For resistant variety,the thickness of upper epidermis greatly increased in low potassium stress ,but the length of upper epidermis ,density of upper epidermis and the ratio of length of upper epidermis to thickness of upper epidermis decreased significantly. At low potassium level, length of the first line palisade cell in tolerant variety reduced. However, length and width of the first and second line palisade cell, thickness of palisade tissue both increased, but the density of palisade cell decreased. Tolerant variety still keep well-developed midvein mesophyll cell and the density of sponge tissue greatly decreased in low potassium stress. The radio of thickness of palisade tissue to thickness of sponge tissue of resistant variety increased significantly in low K level. Compared with CK, the thickness of lower epidermis of tolerant variety increased, while no differences were found for resistant variety.

关 键 词：大豆 耐低钾 叶片显微结构 

分 类 号：S565.1[农业科学—作物学]