木霉菌对大豆幼苗生长的影响及在根部定殖研究  

Effect of Biocontrol Trichoderma on Soybean Seeding and the Growth of MM7 in Soybean Rhizosphere

作　　者：张红骥[1] Allen G. Xue Jinxiu Zhang 许艳丽[2] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]中国科学院东北地理与农业生态研究所,黑龙江哈尔滨150081 [3]Eastern Cereal and OilseedResearch Centre Agriculture and Agri-Food Canada, Ottawa K1AOC6

出　　处：《大豆科学》2009年第3期511-515,共5页Soybean Science

基　　金：2007 MOE-AAFC PhD RESEARCH PROGRAM;哈尔滨科技攻关项目(2005AA3CN179)

摘　　要：分析木霉菌MM35、MM7、MM8、MM9、ZT1、ZT2对大豆幼苗生长的影响,其中MM7、ZT1和ZT2的孢子悬浮液在104cfu.mL-1和105cfu.mL-1的浓度下对大豆发芽不会产生影响,其代谢产物对大豆发芽没有抑制作用。盆栽试验表明:木霉菌MM7不对大豆幼苗产生致病性,且使株高增加13%。盆栽灭菌条件下研究木霉MM7在大豆根部定殖能力表明:木霉菌MM7可随大豆主根和侧根的生长而不断繁殖,且在土壤表面以下0～5cm大豆根际土壤中MM7孢子数量明显高于5～10cm和10～15cm的土层,随着生育时间的延长,不同土层大豆主根和侧根表面木霉菌数量均可在12d～22d之间达到最高值,在根的表面形成保护层,对于大豆苗期病原菌起到一定抑制作用。Fusarium root rots were economically important diseases in soybean in Canada. Biocontrol Trichoderma strains （MM7, ZT1 ,ZT2 , MM8, MM9, MM35 ）are effective against Fusarium root rots. The objective of this research was to evaluate the interaction between biocontrol Trichoderma and soybean growth under laboratory, greenhouse conditions. Trichoderma has no restraint on emerging and seeding growth of soybean ; suspension of MM7, ZT1 and ZT2 will not affect the emergence with the concentration of 10^4cfu. mL^-1 and 10^5cfu.mL^-1 and its metabolic products have no restraint to emergence. Trichoderma MM7 has no pathogenicity on soybean emergence, while it will increase the plant height by 13%. Research of Trichoderrna MM7 colonization on rhizosphere of soybean main root and lateral roots in soil layer of 0-15cm showed that：MM7 can expand vertically with the growth of soybean root;and expand in several directions with the growth of soybean lateral roots, so it formed a＂ protective film＂ at the soybean root. The number of Trichoderma is changing with the growth of soybean roots, and it is higher significantly in soil layer of 0-5cm than other layers and it will reach the maximum amount from 12 to 22 d in main root and lateral roots of different soil layers, which has a certain restraint to soybean root rot in seedling period.

关 键 词：木霉菌(Trichoderma) 大豆幼苗 定殖 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]