岷江百合离体培养快繁体系建立  

Establishment of Rapid Propagation System for Lilium regale in vitro

作　　者：陈丽静[1] 朱首名[1] 张君[2] 张丽[1] 王玉坤[3] 李慧男[1] 马慧[1] 

机构地区：[1]沈阳农业大学生物科学技术学院辽宁省农业生物技术重点实验室,辽宁沈阳110161 [2]辽宁省沈阳市农业检测中心,辽宁沈阳110034 [3]沈阳农业大学植物保护学院,辽宁沈阳110161

出　　处：《辽宁农业科学》2009年第3期12-15,共4页Liaoning Agricultural Sciences

基　　金：辽宁省自然科学基金项目(20072124);辽宁省教育厅重点实验室项目(20060750)

摘　　要：以岷江百合(Lilium regale)为试材,以MS培养基为基础培养基,附加不同种类和浓度的植物生长调节物质(6-BA,NAA,IBA)诱导丛生芽及再生植株。结果表明:采用先用75%的酒精浸泡20 s,再用0.1%Hgcl2浸泡6 min的灭菌方法最为有效,芽增殖最适培养基为MS+6-BA3 mg/L+NAA0.2 mg/L+蔗糖30 g+琼脂7.0 g,可培养出大量具有分化能力的不定芽,芽增殖倍数是最高的。在1/2 MS+NAA 0.1 mg/L+IBA0.01 mg/L上生根质量好,在岷江百合整个培养过程中需要光照,在河沙∶珍珠岩∶草炭土=1∶1∶1基质上,移栽成活率高,可达92%。The influence of different concentration hormone（6-BA, NAA, IBA） for the tissue culture of Lilium regale, based on MS medium were studied in order to explore the suitablest level of hormone and to increase the efficiency of the tissue culture of Lilium regale, and design different components of the four medium to find the best medium, 16ok for best growth conditions, What was more,.transplanting of Lilium regale was studied to establish the high frequency propagation system of Lilium regale. The following were the experimental results through the observation and analysis of the experiment. The best sterilization method was that shoot tip was soaked in the 75% of alcohol for 30s first, and then put in O. 1% mercuric chloride for 6 minutes, Enrich of MS medium with 6-BA 3 mg/L ＋ NAA 0.2 mg/L ＋ sucrose 30 g ＋ agar 7.5 g is the best. promoted the induction of adventitious shoots and their growth. The optimum rooting medium was MS supplemented with NAA O. 1 mg/L ＋ IBA O. Ol mg/L. The Fatshedrea lizei needed light in the process of tissue culture. The transplant survival rate was high on the transplant medium of send： pearlite： peat=l： 1： 1, survival rate was up to 92%.

关 键 词：岷江百合 离体培养 快速繁殖 

分 类 号：Q943.1[生物学—植物学]