猫人参化学成分的气相色谱-质谱联用分析  

Analysis of Chemical Constituents in Roots of Actinidia valvata Dunn with GC-MS Method

作　　者：辛海量[1,2] 徐燕丰[1] 吴迎春[3] 张亚妮[1] 凌昌全[1] 

机构地区：[1]中国人民解放军第二军医大学长海医院,上海200433 [2]中国人民解放军第546医院,新疆乌鲁木齐841700 [3]吉林农业大学中药材学院,吉林长春130118

出　　处：《时珍国医国药》2009年第6期1299-1300,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30572360);上海市科委中药现代化专项基金(No.04DZ19808)

摘　　要：目的研究中药猫人参的低极性化学成分。方法GC-MS的色谱条件:柱温,从60℃开始,保持2min,以20℃/min的速度升到300℃,并保持5min;气化温度为250℃;载气为高纯度氦气,流量1.0ml/min。质谱条件:EI离子源,电子能量70 eV,离子源温度200℃。结果经毛细管色谱分析,共分离出41个峰,并确认其中31种成分,应用色谱峰面积归一法分析各成分的相对含量,结果含量较高的成分有22,23-二溴豆甾醇乙酯(15.20%)、三环[4.1.1.0(2,5)]辛烷(8.58%)、正十六烷酸(6.10%)等。结论猫人参低极性组分复杂,其药理作用有待于深入研究。Objective To investigate the chemical constituents with low polarity in roots of Actinidia valvata Dunn. Methods Constituents with low polarity were identified by gas chromatography - mass spectrometry （ GC - MS） data with Focus DSQ GC - MS system. The GC conditions were as follows ： column, VF - 5ms （ 30m × 0.25mm,0.25μm, VARIAN Company ） ; carrier gas （ N2 ） flow rate, 1.0 ml/min; temperature program, isotherm 2 min at 60℃, 20℃ /min gradient to 300℃, isotherm 5 min; injection temperature, 250℃; detector temperature, 200℃. The EI was 70eV. Results Forty- one chromatographical peaks were separated and 31 chemical constituents were identified, including 22,23- dibromostigmasterol acetate （15.20%）, trieyclo[4.1.1.0（2,5 ）] octane （8.58%） and n -hexadecanoie acid （6.10%） , and so on. Conclusion The chemical composition with low polarity in roots of Aetinidia valvata Dunn is very complicated and its pharmacological action remains to be further studied.

关 键 词：猫人参 化学成分 气相色谱-质谱联用分析 

分 类 号：R284.2[医药卫生—中药学]