微波辅助萃取-分光光度法测定细梗胡枝子叶中总黄酮的含量  

Determination of Total Flavones from Lespedeza virgata (Thunb.) DC. by Microwave-assisted Extraction and Absorption Spectroscopy

作　　者：张帆[1] 齐小花[1] 邹明强[1] 解瑞丽[2] 李锦丰[1] 杨屹[3] 张宏桂[2] 

机构地区：[1]中国检验检疫科学研究院,北京100123 [2]北京中医药大学中药学院,北京100102 [3]北京化工大学理学院,北京100029

出　　处：《时珍国医国药》2009年第6期1323-1324,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家“十一五”科技支撑计划项目(2006BAK10B06)

摘　　要：目的采用微波辅助萃取法提取,以分光光度法测定细梗胡枝子叶中的总黄酮含量。方法单因素法优化微波提取条件为:使用75%乙醇,在液固比70 ml/g下,加热至75℃后持续辐射15 min。结果与传统的乙醇浸提法和超声法相比,微波法具有操作简便,经济可靠,重现性好等特点。结论微波法适合推广应用于各类中草药中总黄酮含量测定的样品前处理。Objective To extract total flavones from Lespedeza virgata （ Thunb. ） DC. by microwave - assisted extraction and determine its content by absorption spectroscopy. Methods The extracted conditions of flavones were optimized as 70 ml/g of 75% ethanol to leaves weight ratio and solution temperature of 75℃ for 15 min. Results Compared with moeeration and ultrasonic -assisted extraction, microwave - assisted extraction was simple and accurate with good reproducibility. Conclusion It is suitable for the determination of flavone in many kinds of traditional Chinese medicines.

关 键 词：微波辅助萃取 分光光度法 细梗胡枝子 总黄酮 

分 类 号：R284.2[医药卫生—中药学]