苦竹叶总黄酮的超声提取及其抗氧化研究  

Extraction of Total Flavanone from Pleioblastus amarus Leaves by Ultrasonic and its Antioxidation Effect

作　　者：黄红英[1] 邓斌[1] 蒋刚彪[2] 

机构地区：[1]湘南学院,湖南郴州423000 [2]华南农业大学,广东广州510642

出　　处：《时珍国医国药》2009年第6期1443-1445,共3页Lishizhen Medicine and Materia Medica Research

基　　金：湖南省教育厅优秀青年科研基金项目(No.06B089)

摘　　要：目的探讨苦竹叶中总黄酮的提取、鉴别方法及对羟自由基的清除能力,以充分利用苦竹叶植物资源,避免资源的浪费。方法采用超声波乙醇浸提法从苦竹叶中提取黄酮类化合物,用分光光度法测定黄酮类化合物的总含量,并利用对羟自由基的清除能力就苦竹叶总黄酮的抗氧化性进行研究。结果测得样品中黄酮类化合物的总含量为C=0.407mg/ml,回收率为100.5%,其纯度和产率均较高。结论该方法采用全物理过程,无任何污染,是提取苦竹叶中黄酮类化合物的有效途径。苦竹叶黄酮类化合物提取液对Fenton体系产生的.OH自由基有很好的清除作用。Objective To study the extraction and identification of total flavanone from Pleioblastus amarus leaves and its effect of scavenging hydroxyl radical. Methods The flavonoids were extracted from pleioblastus amarus leaves with ultrasonic wave and ethanol extraction. Spectrophotometry was used to determine the flavanone content of Pleioblastus amarus leaves. The effect of scavenging hydroxyl radicals of total flavanone from Pleioblastus amarus leaves was also studied. Results By this method, the content of the total flavanone of Pleioblastus arnarus leaves was C = 0. 407mg/ml and the recovery rate was 100.5%. Conclusion This text provides the extraction and purifying methods to get the outcome and the purity of the flavanone is high. This method is a purely physical process and has no pollution. It is an ideal way to extract the total flavanone of Pleioblastus amarus Leaves. Total flavanone from Pleioblastus amarus leaves have scavenging effects on hydroxyl radicals.

关 键 词：苦竹叶 总黄酮 鉴别 超声波提取 清除 自由基 

分 类 号：R284.2[医药卫生—中药学]