促红细胞生成素对新生大鼠缺氧缺血性脑损伤后Bcl-2及VEGF表达的影响  

Effect of exogenous erythropoietin on Bcl-2 and VEGF expression in newborn rats after hypoxia-ischemia brain damage

作　　者：房清丽[1] 阴怀清[1] 孙玲[1] 赵旭晶[1] 

机构地区：[1]山西医科大学第一临床医学院儿科,太原030001

出　　处：《山西医科大学学报》2009年第6期494-497,572,573,共6页Journal of Shanxi Medical University

基　　金：太原市科技局科技兴市专项基金资助项目(0705-026)

摘　　要：目的探讨外源性促红细胞生成素对新生大鼠缺氧缺血性脑损伤(HIBD)后的神经保护作用。方法新生7日龄Wistar大鼠90只随机分为3组:假手术组、HIBD组和EPO治疗组,每组30只。假手术组仅给予颈正中切口游离左侧颈总动脉后缝合切口,不进行缺氧缺血。HIBD组游离并结扎左侧颈总动脉后置于含8%O2的低氧环境中2h制备而成。EPO治疗组,在建立HIBD模型后,即刻一次性给予人基因重组促红细胞生成素(rhEPO)5000U/kg腹腔注射,用HE及免疫组化方法观察术后及给药后不同时间点(6,12,24,48,72h)Bcl-2、VEGF的表达。结果假手术组Bcl-2、VEGF表达弱,HIBD组两者的表达都增加,在同一时间点EPO治疗组与假手术组、HIBD组比较,EPO治疗组两者表达均明显增加(P<0.01)。结论外源性促红细胞生成素可上调新生大鼠HIBD后脑组织Bcl-2、VEGF表达,可能是对脑缺氧缺血保护作用的途径之一。Objective TO explore the neuroprotective effect of erythropoietin in newborn rats following hypoxia-ischemia brain damage （HIBD）. Methods Seven-day-old Wistar rats were randomly divided into three groups （n = 30 in each group） ：sham-operated group, HIBD group, and EPO-treated group. In sham-operated group, the median neck incision was performed, and left common carotid artery was isolated, but not hypoxia-ischemia. In HIBD group,left common cartoid artery was isolated and ligated, then the models were prepared under hypoxia for 2 h. In EPO-treated group, the model rats were instantly given single intraperitoneal injection of human gene recombination erythropoietin（ rhEPO）5 000 U/kg. The expression of Bcl-2 and VEGF was measured after operation for 6 h, 12 h ,24 h ,48 h ,72 h by HE and immunohistochemieal method, respectively. Results Expression of Bel-2 and VEGF was significantly higher in HIBD group than in sham-operated group. Expression of Bcl-2 and VEGF was significantly higher in EPO-treated group than in shamoperated group and HIBD group at the same time （P 〈 0. 01 ）. Conclusion Exogenous erythropoietin can up-regnlate expression of Bcl-2 and VEGF in newborn rats following HIBD ,which may be one of the mechanisms for protecting HIBD.

关 键 词：促红细胞生成素 脑损伤 缺氧缺血 BCL-2 VEGF 神经保护 

分 类 号：R722[医药卫生—儿科]