邻苯二甲酸丁基苄酯对大鼠肝细胞的氧化损伤  

Oxidative Damage of Butyl benzyl phthalate on the Hepatic Cells of Rat

作　　者：秦龙娟[1] 王诗嘉[1] 魏晨曦[1] 程文[1] 胡传禄[1] 杨旭[1] 罗勤[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079

出　　处：《医学研究杂志》2009年第7期34-37,共4页Journal of Medical Research

基　　金：国家科技部卫生部科技支撑计划项目(2006BAI19B05和2006BAJ02A10)

摘　　要：目的研究BBP对大鼠肝脏细胞的作用。方法用不同浓度(5μmol/L,20μmol/L,80μmol/L)的BBP对大鼠肝脏细胞进行体外染毒1h,然后分别用硫代巴比妥酸(Thiobarbituric acid,TBA)法和KCl-SDS沉淀法来检测丙二醛(malondialde-hyde,MDA)含量和DPC系数的变化。结果各染毒浓度下MDA的含量较对照组均显著上升(P<0.01),并且具有很好的剂量-效应关系(R=0.973,P<0.05)。当BBP的浓度为5μmol/L和20μmol/L时,DPC系数与对照组相比略有上升但无显著性差异(P>0.05);当BBP的浓度上升为80μmol/L时,DPC系数明显上升,且与对照组相比有极显著差异(P<0.01)。结论BBP可对大鼠肝脏造成损伤,对机体有毒性作用。Objective To study the effects of BBP on rat hepatic cells. Methods Hepatic cells of rat were exposed to different con- centration of BBP （5 μmol/L, 20μmol/L and 801mol/L） for one hour in vitro. Thiobarbituric acid （TBA） assay and KCI -SDS assay were used to measure the change of the contents of malondialdehyde （MDA） and the coefficient of DNA - protein crosslinks （DPC） , re- spectively. Results That the contents of MDA in hepatic cells of all exposed groups obviously increased compared with the control group （ P 〈 0.01 ） and had a good dose - response relationship （ R = 0. 973, P 〈 0.05 ）. DPC coefficient was increased but without significant difference （P 〉 0.05 ） when under the 5μmol/L and 20μmol/L of BBP. However, DPC coefficient obviously increased compared with the control group （P 〈0.01 ） when the concentration of BBP up to 80μmol/L. Conclnsiou BBP caused the injury to hepatic cells and had toxic effect on the organism.

关 键 词：邻苯二甲酸丁基苄酯 肝细胞 氧化损伤 丙二醛 DNA-蛋白质交联 

分 类 号：R979.1[医药卫生—药品] R994.6[医药卫生—药学]