环境温度对单胺类神经递质微透析探针回收率的影响  

Effect of environment temperature on the recovery of microdialysis probe for monoamine neurotransmitter

作　　者：张恒义[1] 施弘毅[1] 王恩禹[1] 

机构地区：[1]浙江大学生物医学工程与仪器科学学院,浙江杭州310027

出　　处：《浙江大学学报（医学版）》2009年第3期271-275,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省重点科技计划项目(2006C23G2010012)资助

摘　　要：目的:探索微透析探针对单胺类神经递质的回收率受环境温度影响的程度。方法:通过改变微透析探针回收率测量时灌注液温度与待测物质标准液的温度,调节微透析探针的工作环境温度。这分为三种状态,即室温条件:灌注液及标准液温度均调节为室温(24℃);常规条件:灌注液温度调节为室温,标准液温度调节为体温(37.5℃);体温条件:灌注液及标准液温度均调节为体温。用相同的自制4 mm膜长微透析探针分别在三种状态下进行单胺类神经递质去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)混合溶液进行微透析实验,灌注液流速为2μl/min。透析液中单胺类神经递质的含量采用高效液相色谱仪电化学法进行分析,并分别计算微透析探针对三种物质在三种状态下的回收率。结果:在室温条件下,探针对NE的回收率为18.3%,DA的回收率为19.6%,5-HT的回收率为16.9%;在常规条件下,探针对NE、DA、5-HT的回收率分别为29.6%、30.7%、24.3%;在体温组条件下,探针对NE、DA、5-HT的回收率变为49.2%、47.5%、37.2%。方差分析表明,在各温度条件下,探针对三种物质的回收率的总体均数均有显著性差异(P<0.01)。结论:由灌注液温度及其流量和标准液温度决定的微透析探针工作环境温度,显著影响探针对物质的回收率,且对不同物质的影响程度也不同。这提示在进行微透析实验时,为减少温度对微透析探针回收率的影响,要对灌注液、标准液采取适当恒温措施,使微透析探针透析膜工作在恒温环境下进行。Objective： To investigate the effect of environment temperature on the recovery of microdialysis probe for neurotransmitters. Methods： Neurotransmitters NE,DA,and 5-HT were dialyzed with 4 mm membrane length microdialysis probes in different environmental temperature. There were three conditions： lower temperature condition, the temperature of standard solution and that of the perfusate were both 24℃; higher temperature condition,bothwere 37.5 ℃ ; and the middle temperature：the perfusate was 24℃ and the standard solution was 37.5 ℃. The concentrations of neurotransmitters in the dialyzed solution and the standard solution were analyzed by HPLC-ECD,and recoveries were then calculated. Results. In lower temperature condition, the recoveries of microdialysis probe for NE, DA, 5-HT were 18.3%, 19.6% and 16.9%, respectively. In middle temperature condition, the recoveries were 29.6%, 30.7% and 24.3% ,respectively,and in higher temperature condition ,those were 49.2% ,47.5% and 37.2%, respectively. With the analysis of variance, the recoveries for NE, DA, 5-HT increased with temperature significantly （P〈0. 01）. Conclusions： Both the perfusate and the standard solution affects the environmental temperature of microdialysis probe,which in turns affects the recovery of mierodialysis probe for neurotransmitters. So in order to calculate the recovery more accurately,the standard solution/the perfusate should be kept in body temperature.

关 键 词：色谱法 高压液相 神经递质 生物源单胺类/分析 回收率 微透析 温度 

分 类 号：R318[医药卫生—生物医学工程]