树突状细胞联合细胞因子诱导的杀伤细胞对大肠癌细胞株LOVO体外杀伤作用的研究  

Effect of dendritic cells co - cultured with cytokine induced killer cells on cytotoxic activity against colon cancer cells lines LOVO in vitro

作　　者：索冰[1] 王志华[1] 梅芬[1] 

机构地区：[1]哈尔滨医科大学肿瘤防治研究所,哈尔滨150081

出　　处：《实用肿瘤学杂志》2009年第3期219-221,共3页Practical Oncology Journal

摘　　要：目的研究树突状细胞（DC）联合细胞因子诱导的杀伤细胞（CIK）对大肠癌细胞株LOVO的杀伤及诱导凋亡作用。方法从健康供者外周血中提取出单个核细胞，用不同的细胞因子诱导出DC及CIK细胞，待DC成熟后将DC与CIK混合培养。细胞计数法测定细胞的增殖、MTr法测定细胞杀伤活性、透射电镜观察肿瘤细胞的凋亡、WesternBlot法检测效应细胞表面FasL表达。结果DE细胞对CIK细胞具有很强的促增殖作用，且DC—CIK细胞共培养组在各效靶比的杀伤活性均明显高于单独CIK细胞培养组（P〈0．01）。DC可以促进CIK诱导肿瘤细胞发生凋亡，且DC—CIK、CIK细胞均大量表达FasL。结论DC细胞可以显著提高CIK细胞的增殖活性和细胞毒活性，其共培养的作用机制之一可能是通过Fas／FasL途径而实现。Objective To study the cytotoxicity and inducing apoptosis effects of dendritic cells（DC） co - cultured with cytokine induced killer（CIK） cells against colon cancer cells lines LOVO. Methods Mononuclear cells were extracted from health adult peripheral blood. The cells were used to induce DC and CIK respectively with different cytokines, and after DC maturing these two kinds of cells were co - cultured. The proliferation activi- ty of CIK cells were observed by cell counting method, the cytotoxicity was detected by MTT assay, transmission electron microscopy was used to observe the apeptosis of tumor cells, and the expression of FasL in effective cells was determined by Western Blotting method. Results DCs could advance the proliferation activity of CIK cells, and the killing activity of DC - CIK ceils was significant higher than those of CIK cells alone at different effector - target ratios. Transmission electron microscopy showed that DC - CIK could induce colon cells to apoptosis, and quantity expression of FasL on effective cells could be observed. Conclusion DC ceils could obviously enhance the proliferation and cytotoxicity of CIK cells, one of the mechanisms of cytotoxic activity in DC - CIK cells is displayed by Fas/FasL pathway.

关 键 词：树突细胞 细胞因子诱导的杀伤细胞 细胞凋亡 FAS/FASL 

分 类 号：R392.12[医药卫生—免疫学]