鸡输卵管囊肿病的病理学观察及相关病原的初步检测  

Histopathological Observation of Layer Oviduct Cysts and Primary Detection for Correlated Pathogens

作　　者：刘慧敏[1] 邝爱丽[1] 王川庆[1] 陈陆[1] 杨霞[1] 皇甫和平[1] 郑鹿平[1] 徐雪[1] 付仁一[1] 

机构地区：[1]河南农业大学禽病研究所,河南郑州450002

出　　处：《河南农业科学》2009年第6期123-126,130,共5页Journal of Henan Agricultural Sciences

基　　金：中德合作项目(43006167)

摘　　要：为研究卡氏杆菌与鸡输卵管囊肿之间的关系,对42例自然发病鸡进行症状观察、病理剖检,并采取气管、肺脏、输卵管与卵巢组织块,经固定,脱水,石蜡切片,HE染色,光镜观察,利用PCR方法对上述组织进行卡氏杆菌检测,并对其病原混合感染进行了初步研究。结果表明,卡氏杆菌可以引起鸡呼吸道感染和生殖道损伤,显微镜下主要表现为呼吸道黏膜充血、肿胀,输卵管固有层腺体增多,卵巢中卵泡退化,呈空泡状;PCR方法对鸡群卡氏杆菌的阳性检出率为14%,组织阳性检出率为5.4%,且在被检组织中卡氏杆菌很少与其他病原体混合感染。In the present study,the relationship between Gallibacterium anatis and layer oviduct cysts was investigated. The clinical symptoms and gross lesion of 42 layers naturally infected with Gallibacteriurn anatis were observed and recorded. Histopathologic chages of lung, trachea,oviduct and ovary from each bird were observed on rutine paraffin sections stained with HE and detected by PCR for the genus - specificity of Gallibacterium anatis. Additionally, combined infection among Gallibacterium anatis and other pathogens were also investigated. The results showed that Gallibacterium anatis mainly induced the infection in up -respiratory tract and caused lesions in down- gential tract. The hyperemia, swell, epithelium cell degeneration and necrosis were found in the mucosa of respiratory tract,mucosa of the oviduct presented a number of neutrophilic granulocytes and ovarian cysts. The PCR positive detection rate of Gallibacteriurn anatis in layer flocks and tissues was 14% and 5.4%, respectively. Seldom combined infections were observed in the four detected tissues. This indicates that the infection of Gallibacteriurn anatis exists in layer flocks in China and it possibly contributes to the oviduct cysts occurred in layer flocks.

关 键 词：卡氏杆菌 输卵管囊肿 病理学 混合感染 

分 类 号：S852.31[农业科学—基础兽医学]