奶牛体内性控胚胎生产的研究  

Study on the Production of Sexing-embryo in vivo for Dairy Cows

作　　者：张宝龙[1] 施巧婷[2] 杜坤鹏[3] 王伟伟[3] 闫颖颖[3] 张静芳[3] 王新庄[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南省农业科学院畜牧兽医研究所,河南郑州450002 [3]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《河南农业科学》2009年第6期138-140,共3页Journal of Henan Agricultural Sciences

基　　金：国家农业科技成果转化资金项目(2008D00020211)

摘　　要：为探讨奶牛合理的超排方法,采用分离的X冻精对17头超数排卵的荷斯坦奶牛进行人工授精,生产体内性控胚胎。结果表明,处理Ⅰ组在头均获卵数、可用胚及退化胚几个方面都低于处理Ⅱ组,但差异不显著(P>0.05);头均黄体数和未受精数,Ⅰ组高于Ⅱ组(P>0.05);青年牛头均获卵数、可用胚和退化胚方面都高于经产牛组,但差异不显著(P>0.05);青年牛头均未受精卵数显著高于经产牛(P<0.05),头均黄体数低于经产牛,但差异不显著(P>0.05)。In order to approach possible superovulation method, 17 superovulated holstein cattles were inseminated with X - sorted sperm to product sexing - embryos in vivo. The result showed that the average number of embryos collected per cattle, useful embryos and degraded eggs of treatment group Ⅰ is lower than that of group Ⅱ , but no significant difference was observed （P〉0.05）. The number of corpus luteum and unfertilizaed eggs of treatment group Ⅰ is higher than that of group Ⅱ （P〉0.05）. For young cattles,the numbers of embryos,useful embryos and degraded eggs are higher than that of multiparaous cows with no significant difference（P〉0.05）, while the unfertilizaed eggs of group Ⅰ was significant different from that of group Ⅱ （P〈 0.05）. Additionally,the corpus luteum of young cattles was lower than that of multiparaous cows with no significant difference （P〉0.05）.

关 键 词：奶牛 超数排卵 X冻精 性控胚胎 可用胚 

分 类 号：S814.8[农业科学—畜牧学]