Nodosin灌注对离体SD大鼠肝脏组织血红素加氧酶-1表达的影响  

Effect of Nodosin Perfusion on the Expression of HO-1 in Hepar Tissue of SD Rat

作　　者：杜隽铭[1] 孙丽娟[2] 李济宇[1] 刘建文[2] 全志伟[1] 

机构地区：[1]上海交通大学医学院附属新华医院普外科,上海200092 [2]华东理工大学药学院,上海200237

出　　处：《华东理工大学学报（自然科学版）》2009年第3期373-377,共5页Journal of East China University of Science and Technology

基　　金：国家自然科学基金(30600598);上海市青年科技启明星计划(06QA14034);上海交通大学医学院博士创新基金(BXJ0722)

摘　　要：探讨中草药溪黄草有效成分Nodosin对离体SD大鼠肝脏血红素加氧酶-1(HO-1)表达的影响。采用质量浓度为10μg/mL的Nodosin溶液灌注离体SD大鼠肝脏,以乳酸钠林格氏液灌注作为对照,用RT-PCR和Western bloting方法观察两组离体肝脏组织HO-1的蛋白和mRNA表达的变化。实验结果表明:经Nodosin溶液和乳酸钠林格氏溶液灌注保留1 h和2 h,肝脏组织中HO-1在蛋白和mRNA水平上表达较灌注完毕时均有上调(P<0.01),且实验组较对照组上调作用更加明显(P<0.01)。该结果说明溪黄草有效成分Nodosin具有诱导上调离体肝脏组织HO-1表达,从而对大鼠肝移植后缺血再灌注损伤起到有效的保护作用。To examine the effect of nodosin extracted from Isodon serra on heme oxygenase-1 （HO-1） in SD rat hepar in vitro, the isolated liver was perfused by Nodosin solution （10 μg/mL）, while lactic Ringer＇s solution was used as control. Changes in the expression of HO-1 both in the mRNA and protein at 0, 1,2 h after perfusion by Western bloting and RT-PCR were examined. Results show that the expression of HO-1 both in the mRNA and protein is higher in perfused group than that in control, suggesting that Nodosin could induce HO-1 overexpression in isolated SD rat liver and protect hepar tissue by alleviating ischemia incurred by reperfusion.

关 键 词：溪黄草 NODOSIN 血红素加氧酶-1 肝灌注 

分 类 号：R96[医药卫生—药理学]