氟喹诺酮类药物体外诱导大肠埃希菌耐药性变化  

Changes in the drug resistance of E.coli induced by fluoroquinolones in vitro

作　　者：陈妍[1] 罗予 毛理納 

机构地区：[1]郑州市第二人民医院检验科,河南郑州450006 [2]河南省医药科学研究院微生物免疫研究室,河南郑州450052 [3]河南职工医学院药理教研室,河南郑州450000

出　　处：《医学研究生学报》2009年第6期596-599,共4页Journal of Medical Postgraduates

基　　金：河南省教育厅自然科学基础研究计划基金资助项目(批准号:200551288002)

摘　　要：目的：近年来,由于E.coli对氟喹诺酮药物耐药现象日趋严重。文中研究3种氟喹诺酮药物体外诱导大肠埃希菌（E.coli）耐药性,为临床合理使用抗菌药和控制E.coli感染提供指导。方法：采用环丙沙星（CIP）、左氧氟沙星（LEV）和加替沙星（GAT）分别对10株E.coli进行体外分步诱导,采用琼脂平板二倍稀释法测定诱导前后CIP、LEV及GAT对E.coli的最低抑菌浓度（MIC）。结果：E.coli经体外诱导产生获得高水平耐药株（MIC≥128μg/ml）。经1种药物诱导耐药后也不同程度对另外2种药物耐药。结论：大肠埃希菌经氟喹诺酮类药物诱导后MIC变化显著（比诱导前增加8~8205倍）,从体外证实大肠埃希菌对氟喹诺酮类药物逐步耐药的进程。Objective： The drug resistance of E. coli to fluoroquinolones is getting stronger in the recent years. This study aimed at the drug resistance of E. coli induced by fluoroquinolones in vitro, so as to provide a guidance for the rational use of antibiotics and effective control of nosocomial infections. Methods ： Drug resistance was induced in vitro by a multi-step method in 10 strains of E. coli from clin- ical isolates with Ciprofloxacin, Levofloxacin and Gatifloxacin. The minimal inhibition concentration （MIC） of the E. coli was determined by the agar dilution method before and after the induction. Re. sults： After in vitro induction, the E. coli acquired a high resistance （MIC ≥ 128 μg/ml）. And one drug could induce different degrees of resistance to the other two. Conclusion ： The MIC of the E. coli induced by fluoroquinolones in vitro was increased by 8 to 8 205 folds compared with that before induction, which demonstrated a gradually developed resistance of E. coli strains to fluoroquinolones.

关 键 词：大肠埃希菌 氟喹诺酮药物 体外诱导 最低抑菌浓度 

分 类 号：R978[医药卫生—药品]