哺乳期氯氰菊酯暴露对断乳期雄性小鼠生殖内分泌的影响  

Effects of Maternal Cypermethrin Exposure during Lactation on Testicles and Steroidogenesis of Weaning Offspring

作　　者：马兴好[1] 宁萑[1] 王华[1] 万艳梅[1] 王素芳[1] 徐德祥[1] 

机构地区：[1]安徽医科大学公共卫生学院,安徽合肥230032

出　　处：《环境与健康杂志》2009年第6期496-498,F0003,共4页Journal of Environment and Health

基　　金：教育部科学技术研究重点资助项目(208060);安徽省高等学校省级自然科学研究项目(KJ2008B304)

摘　　要：目的探讨哺乳期母鼠接触氯氰菊酯对断乳期雄性子代小鼠生殖内分泌的损害作用,为研究氯氰菊酯的生殖发育毒作用提供理论依据。方法将21只健康清洁级ICR孕鼠随机分为溶剂对照组(玉米油)、低剂量(6.25mg/kg)和高剂量氯氰菊酯染毒组(25mg/kg),每组7只。分娩后第1天,母鼠以灌胃方式进行染毒,每天1次,染毒至分娩后第21天。染毒后,每组随机取雄性仔鼠15只,称重,剖杀取睾丸,称重睾丸并计算脏器系数。采用放免法检测血清、睾丸组织睾酮(T)和血清雌二醇(E2)水平。取小鼠的同侧睾丸,HE染色后进行组织病理学观察。采用TUNEL法检测睾丸细胞凋亡情况。结果与溶剂对照组相比,高、低剂量氯氰菊酯染毒组雄性仔鼠体重、睾丸重量和睾丸系数较低,差异均有统计学意义(P<0.05或P<0.01)。与溶剂对照组相比,高剂量氯氰菊酯染毒组雄性仔鼠血清T水平较低,差异有统计学意义(P<0.01)。与低剂量氯氰菊酯染毒组比较,高剂量氯氰菊酯染毒组雄性仔鼠血清T水平较低,差异有统计学意义(P<0.05)。随着氯氰菊酯染毒剂量的升高,血清和睾丸组织T水平均呈下降趋势。各染毒组雄性仔鼠血清E2和睾丸T水平间比较,差异无统计学意义(P>0.05)。组织病理学结果显示,处理组仔鼠睾丸生精细胞层减少,管腔内径增大,细胞相对稀疏,且排列较紊乱,但睾丸细胞凋亡无明显影响。结论哺乳期母鼠接触氯氰菊酯对断乳期雄性子代小鼠生殖内分泌有明显损害作用。Objective To explore the effects of maternal cypermethrin exposure during lactation on testicle development and steroidogenesis of weaning offspring, and to provide a theoretical basis for the toxicity study of cypermethrin on reproduction. Methods Twenty-one healthy pregnant mice （clean animal） were randomly divided into three groups. Maternal mice were orally administered with different doses of cypermethrin [0,6.25 and 25 mg/（kg.d）, 10 ml/kg] dissolved in corn oil daily from postnatal day I （PNDI） to PND21. Fifteen male pups were randomly selected from each group and sacrificed at PND21 after exposure. The testicle organ coefficients were calculated. Serum testosterone （T） and estrogen （E2）, testicle T were measured by radioimmunoassay （RIA）. Histopathological changes in the testicle tissues were observed by HE stain. Testicle cells apoptosis was detected by TdT-mediated dUTP nick end labeling （TUNEL）. Results A significant decrease was observed in body weight and the testicle organ coefficients in cypermethrin-treated group was in a dose-dependent manner （P〈0.05 or P〈0.01）. Serum T was significantly decreased in pups of high-dose group compared with controls and low-dose group （P〈0.05 or P〈0.01）. Moreover,with the close increasing, the level of serum T and testicular T showed a downward trend. However,there was no significant difference in serum E2 among three groups （P〉0.05）. Histological examination showed that maternal eypermethrin exposure markedly decreased the number and layers of spermatogenic cells, increased the inside diameter （ID） of seminiferous tubules, and disturbed the array of spermatogenie cells in testicle sections of pups at PND21. No significant effect on apoptosis of testicle cells was seen. Conclusion Maternal cypermethrin exposure during Lactation may damage testicles and steroidogenesis of weaning offspring.

关 键 词：农药 氯氰菊酯 哺乳期 睾酮 细胞凋亡 

分 类 号：R994.6[医药卫生—毒理学]