高浓度镍环境中不同培养条件对浸矿微生物群落的影响  

Effect of culture conditions on microbial community with high concentration of nickel ion

作　　者：管昊[1] 尹华群[1] 刘杰[1] 罗焱杰[1] 刘学端[1] 邱冠周[1] 

机构地区：[1]中南大学资源加工与生物工程学院,湖南长沙410083

出　　处：《中南大学学报（自然科学版）》2009年第3期543-549,共7页Journal of Central South University:Science and Technology

基　　金：国家"973"计划项目(2004CB619204);国家自然科学基金资助项目(40646029)

摘　　要：为了解高浓度镍环境中不同能源条件对浸矿微生物群落组成的影响,以59g/L(1mol/L)的镍离子作为选择压力,在不同培养条件下富集酸性环境中的微生物,并通过聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术分析微生物群落多样性。研究结果表明:在高浓度镍离子胁迫下仍存在多种微生物,分别属于变形菌门、酸杆菌和厚壁菌门。此外,研究还发现,不同的富集条件对微生物群落影响很大。当pH值为4时,以亚铁为能源的富集物中,其微生物群落主要以Acidiphilium属和Acidobacterias属为主;在以单质硫为能源的富集物中,90%的微生物属于Acidiphilium;在以硫酸亚铁、单质硫及酵母粉为能源的富集条件下,Acidiphilium和Pseudomonas为优势种群。The influence of energy resources on leaching microbial community was studied. AMD （Acid mine drainage） microbes was cultivated and selected. In high concentration （59 g/L） of nickel sulphate, with different energy resources, the microbial community diversity was analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique. The results show that there are several kinds of bacteria in high-concentration nickel ion, which are close to Firmicutes, Acidobacteria, and Proteobacteria in affinity respectively. Moreover, the results indicate that the different culture conditions show great impact in the microbial community. Acidiphilium and Acidobacterias are the dominant species in microfloras enriched in pH=4 media with ferrous iron as energy sources. In microfloras enriched in pH=4 media with sulfur as energy sources, about 90% of the bacterium are Acidiphilium. With the energy source including ferrous iron, sulfur and yeast extract, Acidiphilium and Pseudomonas are the dominant species.

关 键 词：生物冶金 微生物群落 镍抗性 PCR—RFLP 酸性矿坑水 

分 类 号：Q939[生物学—微生物学]