低碳源浓度条件下聚磷菌特性  

Characteristics of phosphorus accumulating organisms at low carbon level

作　　者：林海[1] 李萍[1] 陈月芳[1] 王倩[1] 侯瑞荣[1] 张艳[1] 

机构地区：[1]北京科技大学土木与环境工程学院,北京100083

出　　处：《北京科技大学学报》2009年第6期679-683,共5页Journal of University of Science and Technology Beijing

基　　金：国家"十一五"科技支撑计划重大资助项目(No.2006BAC19B01)

摘　　要：从北京城市排水集团高碑店污水处理厂二沉池回流污泥中分离获得两株高效聚磷菌株,确定出稀释倒平板分离技术更有利于聚磷微生物的筛选.经形态特征及生理生化实验初步鉴定为不动杆菌属(acinetobacter sp.).确定出A、E两菌的最优碳源选择分别为乙酸钠和谷氨酸钠;并在降低碳源质量浓度,即COD小于100 mg.L-1的条件下进行最佳生长条件优化:A、E两菌的最适pH值分别为8和7,最适温度分别为20℃和25℃;金属镁、钾和锰离子对聚磷微生物具有积极促进作用.聚磷微生物呈现出较好的厌氧放磷、好氧摄磷的特性.Two efficient polyphosphate accumulating organisms, A and E, were screened from the returned sludge which comes from the final sedimentation tank in Beijing Gaobeidian Sewage Treatment Plant. At the same time, the reversing dilution plate separation technology was identified to be beneficial to screening the polyphosphate accumulating organisms. Observations of their morphological, physiological and biochemical features indicate that they are acinetobacter sp. By experiment, the optimal choices for carbon of the polyphosphate accumulating organisms A and E are CH3COONa and C5H8NNaO4, respectively. Experimental data show the best growth conditions of polyphosphate accumulating organisms in reducing carbon concentrations, i.e. less than COD 100 mg·L^-1 ： the optimal pH values and temperatures of the polyphosphate accumulating organisms A and E are 8 and 7, 20 ℃ and 25℃, respec- tively. Metal ions Mg2＋, K ＋ and Mn2 ＋ can be helpful to accumulate polyphosphate. The effect of oxygen on the phosphate accumulating microorganisms was discussed, and the characteristics of P removal in anaerobic condition and P uptake in aerobic condition could be observed.

关 键 词：聚磷菌(PAO) 低碳源 筛选 生物除磷 

分 类 号：X703.1[环境科学与工程—环境工程]