Grb10基因在小鼠胚期特异性表达分析  

The expression analysis of Grb10 during mouse embryonic development

作　　者：刘齐[1] 王燕[2] 陈岩[1] 张凤伟[1] 谷甜甜[1] 曲有鹏[1] 岳磊[1] 吴琼[1] 

机构地区：[1]哈尔滨工业大学生命科学与工程系发育生物学研究室,哈尔滨150001 [2]哈尔滨医科大学附属第一医院妇产科,哈尔滨150001

出　　处：《遗传》2009年第7期732-740,共9页Hereditas（Beijing)

基　　金：教育部留学基金项目(GFEQ24403001);黑龙江省科技计划管项目归国留学基金项目(编号:LC08C05)资助

摘　　要：生长因子受体结合蛋白10(Growth factor receptor-bound protein 10,Grb10)是一个存在于小鼠11号染色体和人7号染色体的母本表达的印记基因。文章利用原位杂交技术和定量RT-PCR方法对不同发育阶段的小鼠胚胎Grb10基因进行时空表达谱的分析,以确定该基因在胚胎发育中其表达与组织发育的关系。定量RT-PCR数据结果表明,Grb10在E8.5-E13.5(Embryonic days8.5-13.5),表达水平逐渐增高,在E13.5达到高峰,后期表达量回落。在胚胎发育的中后期脑、心、肺组织的表达水平呈递减趋势。肝脏组织中Grb10表达水平较为恒定,在E18.5出现高峰。原位杂交数据验证了定量RT-PCR的结果,并且说明了Grb10在其他如骨、肾和肌肉等组织器官的高表达水平。研究结果表明Grb10基因是小鼠胚期发育的一个重要的基因。GrblO gene expression was analyzed in fetus and four organs during gestation process using the real-time RT-PCR and in situ hybridization. The real-time RT-PCR analysis result showed that the expression of GrblO in whole embryos is throughout E8.5 to E19.5. The GrblO gene expression level was gradually increased from E8.5 to E13.5, and then gradually reduced. The tissues specific expression levels were decreased in brain, heart, and lung from E12.5 to E19.5, but a peak expression was observed in liver at E18.5. Grb10 gene expression was also investigated at E13.5, E15.5, E16.5 and E18.5 sections by using in situ hybridization analysis. Strong signals of GrblO were detected in brain, spine, kidney, and muscle tissues. These results suggest that GrblO may play an important role during the development process in mouse.

关 键 词：小鼠 定量RT-PCR Grb10 原位杂交 

分 类 号：Q3[生物学—遗传学]