引种阿斯油橄榄叶中橄榄苦苷提取分离及结构鉴定  

Isolation and Structrual Identification of Oleuropein in Leaves of Ascolano Olive Introduced from Greece

作　　者：王成章[1,2,3] 高彩霞[1,2,3] 叶建中[1,2,3] 陈西娟 姜成英 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室,江苏南京210042 [4]甘肃林业科学研究院,甘肃兰州730000

出　　处：《林产化学与工业》2009年第3期53-57,共5页Chemistry and Industry of Forest Products

基　　金：中国林科院林业新技术研究所基金项目(CAFINT2008C04);国家林业局948项目(2005-4-64)

摘　　要：油橄榄叶主要含有橄榄苦苷、黄酮和多酚等抗氧化物。本研究以引种的希腊油橄榄阿斯为原料,采用乙醇-水(体积比60∶40)对油橄榄叶进行提取,依次用石油醚和乙酸乙酯萃取,乙酸乙酯萃取相经过硅胶柱层析,再用HPLC制备色谱,得质量分数95%以上的单体,通过IR、UV、MS、NMR鉴定该化合物为橄榄苦苷。橄榄苦苷经过酸水解,得到两个主要产物,一个为羟基酪醇,另一个为榄香酸。There are many antioxidants in olive leaves such as oleuropein, flavonoids and polyphenols. Leaves of Ascolano olive introduced from Greece were successively extracted with EtOH-H2O（60：40） , petroleum ether and ethyl acetate. The ethyl acetate fraction was subjected to silica gel column chromatography, then treated with preparative HPLC to obtain a compound with content of 95 %, which was identificated to be oleuropein by UV, IR, MS and NMR. Oleuropein could be acidically hydrolyzed to give hydroxytyrosol and elenolic acid as main .

关 键 词：油橄榄 橄榄苦苷 阿斯油橄榄叶 化学结构 

分 类 号：TQ351.0[化学工程]