超声波提取刺五加主要酚苷及苷元的工艺优化  

Optimized Ultrasonic Extraction of Main Phenolic Glycosides and Aglycones from Acanthopanax senticosus(Rupr. et Maxim.) Harms

作　　者：杨磊[1] 高岩峰[1] 刘洋 郝婧玮[1] 祖元刚[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040

出　　处：《林产化学与工业》2009年第3期93-99,共7页Chemistry and Industry of Forest Products

基　　金："十一五"国家科技支撑计划资助(2006BAD18B04)

摘　　要：采用超声波提取的方法从刺五加根茎中提取紫丁香苷、刺五加苷E和异秦皮啶等有效成分,考察超声波提取时间、乙醇体积分数、料液比和超声波功率等因素对提取的影响。应用Box-Behnken中心组合进行3因素3水平的试验设计,分别以3种目标产物的得率与纯度作为响应值进行工艺优化。响应面法优化后提取工艺条件为:乙醇体积分数59.66%、刺五加与乙醇的料液比1∶6(g∶mL)、提取时间44.9min、超声波功率150W。最佳条件下浸膏中刺五加主要酚苷及苷元的质量分数为2.923%,其中含紫丁香苷1.3%,刺五加苷E1.53%,异秦皮啶0.093%;浸膏得率为6.77%。Ultrasonic-assisted extraction was adopted to extract main phenolic glycosides and aglycones from Acanthopanax senticosus （ Rupr. et Maxim. ） Harms, including the active ingredients such as syringin, eleutheroside E and isofraxidin. According to Box-Behnken central composite experiment design, the extracting process was designed with three factors at three levels. The three factors are volume fraction of ethanol, extraction time and ultrasonic power. The yield and purity of three target products were selected as responses respectively to optimize the extracting process. Response surface analysis （RSA） was applied to determine the effect of central composite experiment design. The optimum conditions of RSA were as follows ： volume fraction of ethanol 59.66 %, ratio of solid to liquid 1 ： 6 （ g ： mL）, ultrasonic time 44.9 min, ultrasonic power 150 W. Under the optimum conditions the content of main phenolic glycoside and aglycone was 2. 923 %, of which the purity of syringin was 1.3 %, isofraxidin was 0. 093 %, eleutheroside E was 1.53 %, extract yield was 6.77 %.

关 键 词：紫丁香苷 刺五加苷E 异秦皮啶 超声波提取 

分 类 号：TQ351.0[化学工程] TQ460.6