超声波强化溶剂提取姜味草中熊果酸和齐墩果酸  

Ultrasonic-assisted Solvent Extraction of Ursolic Acid and Oleanolic Acid from Micromeria biflora Benth.

作　　者：李坤平[1] 潘天玲[1] 贲永光[1] 潘天鸣 

机构地区：[1]广东药学院药科学院,广东广州510006 [2]贵州省黔西南布依族苗族自治州人民医院,贵州兴义562400

出　　处：《林产化学与工业》2009年第3期111-114,共4页Chemistry and Industry of Forest Products

摘　　要：以齐墩果酸和熊果酸得率为指标,对超声波强化溶剂提取姜味草中齐墩果酸和熊果酸工艺进行了研究,利用单因素试验确定提取溶剂,通过正交设计对提取工艺进行优化,并与溶剂回流提取法进行了比较。结果表明,以乙醇为溶剂,超声波强化提取的最佳条件是:体积分数90%乙醇,固液比1∶15,功率为360W,频率40kHz超声波提取2次,每次20min,齐墩果酸和熊果酸总得率为0.732%;回流提取工艺的最优参数是:固液比1∶15,90℃回流提取2次,每次30min,齐墩果酸和熊果酸总得率为0.706%。超声波强化溶剂提取姜味草中熊果酸和齐墩果酸较溶剂回流提取法工艺简单,效率高。Uhrasonic-assisted solvent extraction of oleanolie acid and ursolic acid from Micromeria biflora Benth., was studied and compared with solvent reflux extraction. The solvent was selected based on single factor experiments and the extracting process was optimized by orthogonal test. Using ethanol as solvent, the best results can be achieved under the following conditions： 90 % （volume fraction）ethanol, solid-liquid ratio 1 ： 15, ultrasonic power 360 W at 40 kHz, extracting for 2 times, each time 20 rain, total yield of oleanolic acid and ursolie acid was 0. 732 % ; for solvent reflux extraction the optimal parameters were： solid-liquid ratio 1 ： 15, extracting 2 times at 90℃, each time 30 rain, total yiled of oleanolic acid and ursolic acid was 0.706 %. Ultrasonic-assisted solvent extraction can effectively extract oleanolic acid and ursolic acid from M. biflora with advantages of simple operation and high efficency.

关 键 词：姜味草 齐墩果酸 熊果酸 超声波强化提取 

分 类 号：TQ351.0[化学工程] R284.2[医药卫生—中药学]