超级杂交稻母本OsCHLH cDNA的生物信息学分析与克隆  

Bioinformatics Analysis and Clone of Super Hybrid Rice Female Parental Lines OsCHLH cDNA

作　　者：赵玄之[1] 胡皝[1] 黄志刚[1] 王若仲[1] 萧浪涛[1] 

机构地区：[1]湖南农业大学植物激素与生长发育湖南省重点实验室

出　　处：《激光生物学报》2009年第3期336-340,共5页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(90817101,30670196);湖南省“十一五”重大科技专项子项目(2006NK1001)

摘　　要：通过生物信息学分析得到正确的水稻镁离子螯合酶H亚基（Mg-chelatase H subunit，CHLH）的cDNA。以超级杂交稻母本株1S为材料提取总RNA，并反转录成cDNA，用得到的序列设计特异引物，经PCR扩增出株1ScDNA片段，cDNA片段经T-A克隆后进行测序，获得一条长1350bp序列。提交NCBI的GenBank数据库后接收，登录号为EU569725。Correct CHLH cDNA sequence was obtained from the Oryza sativa by bioinformatics analysis. The extrated from super hybrid rice female parental lines Zhu 1 S was reversely transcripted to eDNA. Specific primers were designed due to the Oryza sativa CHLtt cDNA sequence. After processes such as PCR amplification of the eDNA and T-A clone, a 1 350 bp sequence was obtained, and submitted to Genbank database. Its accession number is EU569725.

关 键 词：脱落酸 OsCHLH 生物信息学分析 株1 S 

分 类 号：S511.01[农业科学—作物学]