草莓茎尖离体快繁体系研究  

Study on Rapid Propagation System of Isolated Stem Apex of Strawberry

作　　者：李金凤[1] 糜林[1] 张计育[2] 王萌[2] 王翡[2] 李国平[1] 

机构地区：[1]江苏丘陵地区镇江农业科学研究所,江苏句容212400 [2]南京农业大学园艺学院,江苏南京210095

出　　处：《江西农业学报》2009年第6期46-47,51,共3页Acta Agriculturae Jiangxi

基　　金：江苏省科技支撑计划(农业)项目(BE2008373);江苏省资源开发局项目(2008kj-046);镇江市科技局项目(NY2008040);江苏省农业科学院科研基金(面上项目)(6110844)

摘　　要：对影响红颊草莓茎尖离体快繁的主要因素进行了研究,结果表明:最适的表面消毒剂为0.1%升汞+2%吐温,未展叶的茎尖为最佳外植体,最适宜的增殖培养基为MS培养基中添加BA 0.5 mg/L和NAA 0.1 mg/L。在此条件下红颊草莓茎尖增殖倍数可达到8.0,且成苗率高达86.3%。This paper researched the main influential factors of rapid propagation system of strawberry in vitro. The result indicated that the preferable exterior disinfector was 0. 1% Hg ＋2% tween, the best explant was the stem apex without leaf, the optimal propagation medium was MS ＋ BA 0.5 mg/L ＋ NAA 0. 1 mg/L. Under these conditions, the proliferous times of the isolated stem apex of strawberry could attain 8.0, and the seedling - becoming rate could achieve 86.3%.

关 键 词：红颊草莓 茎尖 快速繁苗 增殖倍数 

分 类 号：S668.4[农业科学—果树学]