高效液相色谱法测定人血浆中万古霉素质量浓度  

Determination of Vancomycin in Human Plasma by HPLC

作　　者：杜贯涛[1] 谢小菊[1] 刘广军[1] 翁春梅[1] 

机构地区：[1]江苏省常州市第二人民医院药剂科,江苏常州213003

出　　处：《中国药业》2009年第13期17-18,共2页China Pharmaceuticals

摘　　要：目的建立测定血浆样品中万古霉素浓度的高效液相色谱(HPLC)法。方法色谱柱为菲罗门双子星C18柱(150mm×4.6mm,5μm)流动相为50mmol/L磷酸二氢钾溶液-乙腈(91.0∶9.0),流速为1.0mL/min,检测波长为230nm,柱温30℃。结果万古霉素质量浓度在5.0～100.0μg/mL范围内与峰面积线性关系良好(r=0.9998),最低检测质量浓度为0.2μg/mL;日内、日间精密度RSD分别为3.1%～3.6%和3.5%～5.6%(n=5);低、中、高3种质量浓度净化回收率分别为64.9%,66.3%,65.6%(n=3),方法回收率分别为100.6%,100.2%,98.9%(n=5)。结论HPLC法快速、简便,结果准确,可用于万古霉素血药浓度监测及体内药代动力学研究。Objective To establish a HPLC method to determine the vancomycin content in human plasma. Methods The column was Phenomenex C18 column （150 mm × 4.6 mm, 5 μm）, the mobile phase was mixture of 50 mmol/L potassium dihydrogen phosphate -acetonitrile （91.0：9.0）. The flow rate was 1.0 mL/min. UV detection was set at 230 nm and the column temperature was 30 ℃. Results The calibration curve was linear within the range of 5.0- 100.0 μg/mL（ r=0. 999 8）. The limit of test was 0. 2 μg/mL. Intra-day and inter-day RSD were 3.1% - 3.6% and 3.5% - 5.6% （ n = 5） respectively. The purification recovery rates were 64.9%, 66.3% and 65.6% （ n = 3）, and the method recovery rates were 100. 6%, 100. 2%, 98.9% （n =5）, respectively. Conclusion The method is rapid, simple and accurate. It can be used in blood concentration monitoring and pharmacokinetics study of vancomycin.

关 键 词：万古霉素 高效液相色谱法 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R978.69[医药卫生—药学]