磁性附着体中铁素体不锈钢引起小鼠成纤维细胞L929的死亡模式(英文)  

Death patterns of the mouse fibroblast cell L929 induced by ferrite stainless steel in magnetic attachment

作　　者：郭传波[1] 战德松[2] 

机构地区：[1]沈阳市口腔医院,辽宁省沈阳市110002 [2]中国医科大学附属口腔医院,辽宁省沈阳市110002

出　　处：《中国组织工程研究与临床康复》2009年第25期4821-4824,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：the Scientific and Technological Research Projects of Liaoning Province, No 2004225003-2~~

摘　　要：背景:作为自制磁性附着体外包裹材料的26-1超纯铁素体不锈钢在以往的实验中已初步证实其具有良好的生物学特性,但选用凋亡机制对其生物相容性进行深入研究和评价的报道较少。目的:应用L929细胞从分子生物学水平探讨26-1超纯铁素体不锈钢引起细胞的死亡模式,评价26-1超纯铁素体不锈钢是否符合临床应用的生物相容性要求。设计、时间及地点:分子生物学水平,对比观察实验,于2004-06/2005-06在中国医科大学中心实验室完成。材料:26-1超纯铁素体不锈钢(超低碳,名义成分为Fe-26%Cr-1%Mo),Tilite(含钛医用合金,名义成分为70%Ni-16%Cr-(4%～6%)Ti-其他合金元素)、Co-Cr合金(名义成分为Co-30%Cr-5%Mo)。利用蜡型铸造制备实验用材料,并将材料分别切割成直径12mm的圆盘样品。方法:①将3种材料与L929细胞联合培养,阴性对照组为RPMI1640正常培养细胞,孵育18h和24h后应用异硫氰酸荧光素标记的膜联蛋白结合碘化丙啶染色双参数技术对细胞死亡类型进行定量检测。②25%,50%,100%浓度的3种材料浸提液体外培养小鼠成纤维细胞L92924h后,采用流式细胞术碘化丙啶染色法检测L929细胞的凋亡率及细胞周期分布情况。主要观察指标:①不同材料引起L929细胞凋亡和坏死的比例。②L929细胞凋亡率及细胞周期分布情况。结果:各材料组均降低了L929细胞的生存率,其中细胞坏死水平在各材料组与阴性对照组之间差异无显著性意义,说明引起的致细胞病变效应主要是由细胞凋亡引起的,各材料组之间产生的效应差异无显著性意义。并且L929细胞凋亡具有明显的浓度依赖性及时间依赖性。3种实验用金属材料引起的L929细胞凋亡率在各浓度组间差异无显著性意义。结论:26-1超纯铁素体不锈钢引起细胞的死亡方式主要是凋亡,符合临床应用材料的生物相容性要求。BACKGROUND： It has been reported that 26-1 ferrite stainless steel has excellent biological characteristics, but the report of its biocompatibility is rare, especially the evaluation of biocompatibility by apoptosis mechanism. OBJECTIVE： To study the pattern of cell death induced by magnetic-attached 26-1 ferrite stainless steel and to evaluate whether 26-1 ferrite stainless steel meets the biocompatibility in clinical application. DESIGN, TIME AND SETTING： A contrast observational study at the level of molecular biology was performed at Central Laboratory of China Medical University from June 2004 to June 2005. MATERIALS： 26-1 ferrite stainless steel （Fe - 26% Cr - 1% Me）, Tilite [70% Ni - 16% Cr - （4%-6%） Ti - other alloy elements] and Co-Cr alloy （Co - 30% Cr - 5% Mo） were used in this study. Embed and cast metal materials then were cut into roll discs whose diameters were 12 mm. METHODS： （1） The fibroblast L929 cells were co-cultured with the three materials, while cells in the negative control group were cultured in RPMI1640 media. At 18 and 24 hours after culture, types of cell death were quantitatively detected using fluorescein isothiocyanate and propidium iodide （PI） staining. （2） Apoptosis rate and cycle distribution of L929 cells were detected using flow cytometry and PI staining at 24 hours after respectively culturing in 25%, 50%, and 100% leaching liquor. MAIN OUTCOME MEASURES： Ratio between apoptosis and necrosis of L929 cells induced by different materials; apoptotic rate and cycle distribution of L929 cells. RESULTS： All the three materials could decrease the survival rate of L929 cells; in particular, there was no significant difference in necrosis rate between different concentration material groups and negative control group, suggesting that cell apoptosis was the major factor to induce cytopathic effect, but there was no significant difference between the three material groups. Apoptosis of L929 cells indicated a great fashion of time- and concentration-d

关 键 词：磁性附着体 铁素体不锈钢 凋亡 成纤维细胞L929 

分 类 号：R318[医药卫生—生物医学工程]