棉花γ-生育酚甲基转移酶基因全长cDNA的克隆与序列分析  

Cloning and Sequence Analysis of γ-tocopherol Methyltransferase (γ-TMT) cDNA from Cotton (Gossypium hirsutum)

作　　者：邹礼平[1] 高和平[1] 

机构地区：[1]孝感学院生命科学技术学院,湖北孝感432000

出　　处：《江苏农业学报》2009年第3期490-493,共4页Jiangsu Journal of Agricultural Sciences

基　　金：孝感学院博士启动基金资助课题(2006BQ02)

摘　　要：γ-生育酚甲基转移酶（γ-TMT）是植物维生素E生物合成途径中的关键酶。通过RACE—PCR得到了棉花γ-TMT基因的cDNA序列，全长1384bp，包含一个长为1035bp的完整开放读码框，推导的氨基酸序列与番茄、大豆、拟南芥、向日葵、玉米等植物的γ-生育酚甲基转移酶基因编码的氨基酸序列的同源性较高，序列的一致率为68％～81％；与莱茵衣藻的序列同源性较低，一致率只有54％。系统进化分析结果表明不同来源的γ-TMT基因聚为3类。γ-tocopherol methyhransferase （γ-TMT） is a key enzyme of vitamin E biosynthesis in plant. The eDNA of cotton γ-TMT was cloned by RACE-PCR with primers designed based on a EST （DT544964） sequence. The full-length of cDNA sequence was 1 384 bp, contained a complete open reading frame （ORF） of 1 035 bp, which encoding 344 amino acid residues. Homology analysis of the deduced amino acid showed 68 % - 81% identity with γ- TMT from tomato, soybean, Arabidopsis thaliana, sunflower and maize, and only 54% identity with γ-TMT from Chlamydomonas reinhardtii. The result of phylogenetic analysis indicated that various γ-TMT sequences were clustered into three groups.

关 键 词：棉花 Γ-生育酚甲基转移酶 维生素E 生物合成 基因克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]